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Tsa biotin plus system

Manufactured by PerkinElmer
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The TSA-Biotin Plus System is a laboratory equipment product designed for use in various applications that require signal amplification. It provides a method for enhancing the detection of low-abundance targets, enabling more sensitive and reliable analysis.

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Lab products found in correlation

Rnf125, by Merck Group (2 mentions) P stat3, by Cell Signaling Technology (2 mentions) Hpa041514, by Merck Group (2 mentions) Rnf125, by Cell Signaling Technology (2 mentions)

Close spelling variants of this product

TSA Biotin System (29 citations) TSA Plus System (14 citations) TSA system (13 citations) TSA Plus Biotin (2 citations) TSATM Biotin System (2 citations)

2 protocols using tsa biotin plus system

1

Immunohistochemical Analysis of RNF125 in Melanoma

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Tumor biopsies were stained with primary antibodies for RNF125 (HPA041514; Sigma-Aldrich) followed by a secondary antibody conjugated to horseradish peroxidase and treated with 3,3′-diaminobenzidine (DAB) chromogen. Stained slides were interpreted by a dedicated dermatopathologist. Only positive signals with clear melanoma morphology were evaluated. For immunofluorescence, the primary antibodies were RNF125 and pSTAT3 (both diluted 1:1,000; Cell Signaling), and signals were amplified using the TSA-Biotin Plus System (Perkin Elmer) according to the manufacturer’s instructions. The same slides were counterstained with S-100 (1:400; Dako). Immunofluorescence-stained slides were visualized using a fluorescence microscope with a Slidebook or Aperio slide scanner.
Kim H., Frederick D.T., Levesque M.P., Cooper Z.A., Feng Y., Krepler C., Brill L., Samuels Y., Hayward N.K., Perlina A., Piris A., Zhang T., Halaban R., Herlyn M.M., Brown K.M., Wargo J.A., Dummer R., Flaherty K.T, & Ronai Z.A. (2015). Downregulation of the Ubiquitin Ligase RNF125 Underlies Resistance of Melanoma Cells to BRAF Inhibitors via JAK1 Deregulation. Cell reports, 11(9), 1458-1473.
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2

Immunohistochemical Analysis of RNF125 in Melanoma

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumor biopsies were stained with primary antibodies for RNF125 (HPA041514; Sigma-Aldrich) followed by a secondary antibody conjugated to horseradish peroxidase and treated with 3,3′-diaminobenzidine (DAB) chromogen. Stained slides were interpreted by a dedicated dermatopathologist. Only positive signals with clear melanoma morphology were evaluated. For immunofluorescence, the primary antibodies were RNF125 and pSTAT3 (both diluted 1:1,000; Cell Signaling), and signals were amplified using the TSA-Biotin Plus System (Perkin Elmer) according to the manufacturer’s instructions. The same slides were counterstained with S-100 (1:400; Dako). Immunofluorescence-stained slides were visualized using a fluorescence microscope with a Slidebook or Aperio slide scanner.
Kim H., Frederick D.T., Levesque M.P., Cooper Z.A., Feng Y., Krepler C., Brill L., Samuels Y., Hayward N.K., Perlina A., Piris A., Zhang T., Halaban R., Herlyn M.M., Brown K.M., Wargo J.A., Dummer R., Flaherty K.T, & Ronai Z.A. (2015). Downregulation of the Ubiquitin Ligase RNF125 Underlies Resistance of Melanoma Cells to BRAF Inhibitors via JAK1 Deregulation. Cell reports, 11(9), 1458-1473.
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