The TRPV4 antagonist HC067047, agonist GSK1016790A and rottlerin were purchased from Abcam (UK). The TRPV4 agonist GSK1016790A (3 μg/kg body weight), TRPV4 antagonist HC067047 (0.5 mg/kg body weight), PKC-δ inhibitor rottlerin (0.3 mg / kg body weight), CaCl2 (80 mg/kg body weight), EDTA (40 mg/kg body weight), EGTA (130 mg/kg body weight) were intravenously injected via the tail vein. The doses of the above-listed chemicals were selected based on previous reports [30] (link), [31] (link), [32] (link), [33] (link), [34] (link), [35] . The GSK1016790A, HC-067047, and rottlerin were first dissolved in DMSO, and then in normal saline to a final volume of 1 ml with a DMSO concentration of 1%. The control groups were treated correspondingly with saline containing the same DMSO dose.
Hc067047
Manufactured by Abcam
Sourced in United Kingdom
The HC067047 is a lab equipment product. It is a 50 ml Conical Centrifuge Tube made of polypropylene material. The tube is designed for general laboratory use, such as sample preparation, centrifugation, and storage. The product specifications and details are available upon request.
Automatically generated - may contain errors
Lab products found in correlation
Rottlerin, by Abcam (1 mentions)
Ketoconazole, by Merck Group (1 mentions)
Dilauroyl phosphatidylcholine, by Fujifilm (1 mentions)
β nadph, by Oriental Yeast (1 mentions)
Fluo 4 am, by AAT Bioquest (1 mentions)
Capsazepine, by Cayman Chemical (1 mentions)
Arachidonic acid sodium salt, by Merck Group (1 mentions)
Nerve growth factor ngf, by Alomone (1 mentions)
Fetal calf serum (fcs), by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Fujifilm (1 mentions)
Penicillin streptomycin solution, by Merck Group (1 mentions)
Fura 2 am, by Abcam (1 mentions)
Horse serum, by Equitech-Bio (1 mentions)
2 protocols using hc067047
1
Pharmacological Modulation of TRPV4 in Vivo
2
Cytochrome P450 Enzyme Activity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dulbecco's modified Eagle's medium (DMEM), Dilauroyl‐ phosphatidylcholine, and RN‐1747 were purchased from Wako Pure Chemicals (Osaka, Japan). Fetal calf serum (FCS), penicillin‐ streptomycin solution, ketoconazole, and arachidonic acid sodium salt were purchased from Sigma Chemical (St. Louis, MO). 5,6‐, 8,9‐, 11,12‐, and 14,15‐EET, 5‐, 8‐, 9‐, 11‐, 12‐, 15‐, 16‐, 17‐, 18‐, 19‐, and 20‐HETE, and capsazepine were purchased from Cayman Chemical Co. (Ann Arbor, MI). Antibodies against CYP2C11, 2C23, 2E1, 4A2, sEH, NADPH‐cytochrome P450 reductase, and β‐actin were prepared as described previously.16 , 17 , 18 β‐NADPH was purchased from Oriental Yeast Co. LTD (Tokyo, Japan), horse serum was from Equitech‐Bio (Kerrville, TX), and nerve growth factor (NGF) was form Alomone Labs (Jerusalem, Israel). HC067047 and Fura‐2 AM were from Abcam plc (Cambridge, UK). Fluo‐4AM was from AAT Bioquest (Sunnyvale, CA).
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