Oligonucleotides comprising the circ_0084927 mutant (the sequence containing the miR-1179 binding site was mutated to GAUACGA) or the CDK2 mRNA 3′UTR mutant (the sequence containing the miR-1179 binding site was mutated to GAUACGA) were synthesized by GenePharma (Shanghai, China). Inserted into the dual-luciferase miRNA target expression vector (pGL4) were wild-type circ_0084927, circ_0084927 mutant, CDK2 3′UTR mutant, and wild-type CDK2 3′UTR. This insertion was performed to construct luciferase reporter plasmid. HeLa and C-33A cells were also co-transfected with luciferase porter plasmid and miR-1179 mimic. After 48 h of incubation, the culture medium was removed to collect the cells. The collected cells were then lysed to obtain cell lysates. The luciferase activity was measured by Pierce Renilla-Firefly Luciferase Dual Assay Kit (16185; Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the protocol.
Cdk2 mrna 3 utr mutant
Manufactured by GenePharma
Sourced in China
CDK2 mRNA 3'UTR mutant is a lab equipment product that allows for the study of the 3' untranslated region (3'UTR) of the CDK2 gene. The 3'UTR plays a role in the regulation of gene expression. This product can be used to investigate the impact of mutations in the CDK2 mRNA 3'UTR.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using cdk2 mrna 3 utr mutant
1
Validation of miR-1179 Binding Sites
2
Regulation of circ_0084927 and CDK2 by miR-1179
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oligonucleotides comprising the circ_0084927 mutant (the sequence containing the miR-1179 binding site was mutated to GAUACGA) or the CDK2 mRNA 3'UTR mutant (the sequence containing the miR-1179 binding site was mutated to GAUACGA) were synthesized by GenePharma (Shanghai, China). circ_0084927 wild type, circ_0084927 mutant, CDK2 3′UTR mutant and CDK2 wild-type were inserted into the Dual-Luciferase miRNA target expression vector (pGL4) to construct luciferase reporter plasmid. HeLa and C-33A cells were co-transfected with luciferase porter plasmid and miR-1179 mimic. After 48 hours of incubation, the culture medium was removed to collect the cells. The collected cells were lysed to collect cell lysates. The luciferase activity was measured by Pierce Renilla-Fire y Luciferase Dual Assay Kit (16185; Thermo Fisher Scienti c, Inc., Waltham, MA, USA) according to the protocol.
3
Validation of miR-1179 Binding Site Mutations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oligonucleotides comprising the circ_0084927 mutant (the sequence containing the miR-1179 binding site was mutated to GAUACGA) or the CDK2 mRNA 3'UTR mutant (the sequence containing the miR-1179 binding site was mutated to GAUACGA) were synthesized by GenePharma (Shanghai, China). Inserted into the dual-luciferase miRNA target expression vector (pGL4) were wild-type circ_0084927, circ_0084927 mutant, CDK2 3′UTR mutant, and wild-type CDK2 3'UTR. This insertion was performed to construct luciferase reporter plasmid. HeLa and C-33A cells were also co-transfected with luciferase porter plasmid and miR-1179 mimic. After 48 hours of incubation, the culture medium was removed to collect the cells. The collected cells were then lysed to obtain cell lysates. The luciferase activity was measured by Pierce Renilla-Fire y Luciferase Dual Assay Kit (16185; Thermo Fisher Scienti c, Inc., Waltham, MA, USA) according to the protocol.
4
Regulation of circ_0084927 and CDK2 by miR-1179
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oligonucleotides comprising the circ_0084927 mutant (the sequence containing the miR-1179 binding site was mutated to GAUACGA) or the CDK2 mRNA 3'UTR mutant (the sequence containing the miR-1179 binding site was mutated to GAUACGA) were synthesized by GenePharma (Shanghai, China). circ_0084927 wild type, circ_0084927 mutant, CDK2 3′UTR mutant and CDK2 wild-type were inserted into the Dual-Luciferase miRNA target expression vector (pGL4) to construct luciferase reporter plasmid. HeLa and C-33A cells were co-transfected with luciferase porter plasmid and miR-1179 mimic. After 48 hours of incubation, the culture medium was removed to collect the cells. The collected cells were lysed to collect cell lysates. The luciferase activity was measured by Pierce Renilla-Fire y Luciferase Dual Assay Kit (16185; Thermo Fisher Scienti c, Inc., Waltham, MA, USA) according to the protocol.
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