Black filter paper

Manufactured by Merck Group

Sourced in Morocco

Black filter paper is a type of laboratory equipment used for filtration. It is made of cellulose fibers and is designed to remove solid particles from liquids or gases. The black color is achieved through the addition of carbon or other pigments during the manufacturing process. The primary function of black filter paper is to provide a high-contrast surface for visual inspection and analysis of the filtered material.

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Lab products found in correlation

Hyclone dulbecco s modified eagle s medium, by GE Healthcare (1 mentions) Cryostat, by Leica (1 mentions) Cm3050s cryostat, by Leica (1 mentions)

2 protocols using black filter paper

Preparation and Fixation of Retinal Samples

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The eyes were dissected in refrigerated HyClone Dulbecco’s Modified Eagles Medium (GE Healthcare Life Sciences, Logan, UT). The eyecups were then immersion-fixed in 4% (w/v) paraformaldehyde (PFA) in 0.1 M phosphate buffered saline (PBS), pH 7.4 for 45 minutes to 1 hour and cryoprotected overnight in 30% sucrose. These eyecups were sectioned at 16–20 μm with a Leica cryostat (Leica Microsystems, Buffalo Grove, IL) and mounted onto slides, which were stored at -20°C. For whole-mounted retinas, the sclera was removed and the retina was flattened photoreceptor side down on black filter paper (EMD Millipore Corporation, Bedford, MA). The retina was subsequently immersion-fixed in 4% PFA in 0.1 M PBS for 1 hour. The whole-mounted retina was stored in 0.1 M PBS until processing for immunohistochemistry.

Solomon A.M., Westbrook T., Field G.D, & McGee A.W. (2018). Nogo receptor 1 is expressed by nearly all retinal ganglion cells. PLoS ONE, 13(5), e0196565.

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Monkey Retina Cryosectioning and Immunolabeling

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The eyecups were fixed in 4% paraformaldehyde (PFA) in 0.1 M phosphate buffer (PB), pH 7.4, for 15–60 minutes and stored in 30% sucrose in 0.1 M PB overnight at 4°C. The monkey retina (UC1) from UCLA was fixed 30 minutes in 4% PFA and stored in 0.1 M PB at 4°C and the monkey retina from OSHU was fixed for 60 minutes in 4% PFA and stored in 30% sucrose in PB saline, and shipped to UCLA. For cryostat sections, the eyecup or isolated retina were sectioned vertically at 12 μm using a Leica CM3050S cryostat (Leica Microsystems, Buffalo Grove, IL) and collected onto gelatin-coated slides and stored frozen until immunohistochemistry processing. For whole-mounted retinas, the retina was separated from the sclera and mounted photoreceptor side down on black filter paper (EMD Millipore, Billerica, MA). Retinas were fixed in 4% PFA in 0.1 M PB for 15 minutes, and transferred to the blocking solution as described above for 12–16 hours overnight at 4°C before immunohistochemistry processing.

Rodriguez A.R., de Sevilla Müller L.P, & Brecha N.C. (2014). The RNA binding protein RBPMS is a selective marker of ganglion cells in the mammalian retina. The Journal of comparative neurology, 522(6), 1411-1443.

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