Escherichia coli DH5α was purchased from TIANGEN (Beijing, China). P. pastoris X-33 and expression plasmid pPICZαA were conserved at −80 °C. EcoRІ, KpnІ, SacI, and universal DNA purification kits were purchased from TaKaRa (Beijing, China). Buffered glycerol-complex medium (BMGY), buffered methanol-complex medium (BMMY), and yeast extract peptone dextrose (YPD) were prepared based on the protocols provided by Invitrogen (Carlsbad, CA, USA).
Buffered glycerol complex medium
Manufactured by Thermo Fisher Scientific
Sourced in United States
Buffered glycerol-complex medium is a culture medium used for the growth and maintenance of microbial cells. It provides a balanced source of nutrients, including glycerol and other complex components, to support cellular metabolism and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Yeast extract peptone dextrose, by Thermo Fisher Scientific (1 mentions)
Escherichia coli dh5α, by Tiangen Biotech (1 mentions)
Yeast extract peptone dextrose medium, by Thermo Fisher Scientific (1 mentions)
Guaiacol, by Merck Group (1 mentions)
P pastoris gs115, by Thermo Fisher Scientific (1 mentions)
2 6 dimethoxyphenol 2 6 dmp, by Merck Group (1 mentions)
Ppic9k, by Thermo Fisher Scientific (1 mentions)
Escherichia coli jm109, by Thermo Fisher Scientific (1 mentions)
2 protocols using buffered glycerol complex medium
1
Recombinant Protein Expression in P. pastoris
2
Heterologous Enzyme Expression in P. pastoris
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Escherichia coli JM109, P. pastoris GS115 and the plasmid pPIC9K were purchased from Invitrogen (Carlsbad, CA, USA). Guaiacol, 2,6-dimethoxyphenol (2,6-DMP), and 2,2′-azino-bis(3-ethylbenzothazoline-6-sulfonate) (ABTS) were obtained from Sigma-Aldrich (St. Louis, MO, USA). The error-prone PCR kit was acquired from Aviva Systems Biology (Beijing, China). All other chemicals and reagents were of analytical grade. Yeast Extract Peptone Dextrose Medium (YPD), Minimal Dextrose Medium (MD), Buffered Glycerol-Complex Medium (BMGY) and Buffered Minimal Methanol Medium (BMM) were prepared following the instructions from Invitrogen.
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