Caco-2 human epithelial colorectal adenocarcinoma cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with Gibco® 10% fetal bovine serum (FBS; Thermo Fisher Scientific). LDH-DCA-HA and INS@LDH-DCA-HA were each incubated with Caco-2 cells for 24 h. Thereafter, 10 μL MTT reagent (Beyotime, Shanghai, China) was added to the medium, according to the manufacturer’s instructions, and the optical density (OD) was measured using an enzyme-labeled instrument (TECAN, Geneva, Switzerland).
Gibco 10 fetal bovine serum fbs
Manufactured by Thermo Fisher Scientific
Sourced in United States
Gibco® 10% fetal bovine serum (FBS) is a cell culture supplement derived from fetal bovine blood. It provides a source of proteins, growth factors, and other nutrients essential for the growth and maintenance of various cell types in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Enzyme labeled instrument, by Tecan (1 mentions)
Mtt reagent, by Beyotime (1 mentions)
Caco 2 human epithelial colorectal adenocarcinoma cells, by American Type Culture Collection (1 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Hyclone dmem high glucose medium, by Thermo Fisher Scientific (1 mentions)
Co2 incubator, by Thermo Fisher Scientific (1 mentions)
Gibco dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Ges 1, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)
Bgc 823, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)
Sgc 7901, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)
Mkn45, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)
3 protocols using gibco 10 fetal bovine serum fbs
1
Caco-2 Cytotoxicity Assay with LDH-DCA-HA
2
Primary CRC cell cultivation
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After obtaining written informed consent, primary cells were isolated from 12 CRC patients treated at Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai TCM University (Shanghai, China). The cells were cultured in a 5% CO2 incubator (Thermo Fisher Scientific, Inc., Waltham, MA, USA) at 37°C with HyClone™ high glucose DMEM medium (cat. no. SH30243.01; Thermo Fisher Scientific, Inc.) supplemented with Gibco™ 10% fetal bovine serum (FBS; cat. no. 16000-044; Thermo Fisher Scientific, Inc.) and 1% antibiotic (penicillin and streptomycin; cat. no. P1400-100; Beijing Solarbio Science & Technology Co., Ltd., Beijing, China). The medium was refreshed every two days during culture.
3
Clinical Gastric Cancer Tissue Samples
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Clinical samples. A total of 57 gastric cancer and matched, non-tumorous gastric tissue samples were collected from patients of the The Affiliated Huai'an Hospital of Xuzhou Medical University (Huai'an, China) and The Second People's Hospital of Huai'an (Huai'an, China) between 2011 and 2014. None of the gastric cancer patients received chemotherapy or radiotherapy treatments prior to surgery. All tissue samples were immediately frozen in liquid nitrogen and stored at -80˚C. The seventh edition of UICC tumor node metastasis (TNM) classification system was used as clinical staging criteria (18) . The present study was approved by the Ethics Committee of The Affiliated Huai'an Hospital of Xuzhou Medical University and The Second People's Hospital of Huai'an, and written informed con sent was obtained from all gastric cancer patients.
Cell culture. Five gastric cancer cell lines (SGC-7901, BGC-823, AGS, MKN-1 and MKN-45) and a normal gastric epithelium cell line (GES-1) were purchased from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China) and cultured in Gibco Dulbecco's modified Eagle's medium (DMEM; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with Gibco 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Inc.) at 37˚C in an atmosphere consisting of 5% CO 2 .
Cell culture. Five gastric cancer cell lines (SGC-7901, BGC-823, AGS, MKN-1 and MKN-45) and a normal gastric epithelium cell line (GES-1) were purchased from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China) and cultured in Gibco Dulbecco's modified Eagle's medium (DMEM; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with Gibco 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Inc.) at 37˚C in an atmosphere consisting of 5% CO 2 .
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