Nb100 304

Manufactured by Fortis Life Sciences

Sourced in Spain

The NB100-304 is a laboratory instrument designed for biological sample analysis. It features a compact design and can be used for various applications in life sciences research. The core function of the NB100-304 is to provide accurate and reliable measurements of samples.

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Lab products found in correlation

Clone jbw301, by Merck Group (1 mentions) Nb100 143, by Novus Biologicals (1 mentions) Clone 7f9, by Merck Group (1 mentions) Prolong diamond anti fade mount, by Thermo Fisher Scientific (1 mentions) Ab2893, by Abcam (1 mentions)

2 protocols using nb100 304

Immunofluorescence Assay for DNA Damage Response Proteins

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Mouse monoclonal anti-γH2AX (Ser139) (clone JBW301; 1:500; Millipore, 05-636); rabbit polyclonal anti-γH2AX (Ser139) (1:300; Novus Biological, NB100-384); rabbit polyclonal anti-γH2AX (Ser139) (1:500; Abcam, ab11174); rabbit polyclonal anti-NBS1 (1:300; Novus Biological, NB100-143); rabbit polyclonal anti-53BP1 (1:1000; Novus Biological, NB100-304); rabbit polyclonal anti-53BP1 (1:1000; Bethyl A300-272A); mouse monoclonal anti-MDC1 (clone mdc1-50; 1:400; Sigma, M2444); sheep polyclonal anti-MDC1 (#3035; 1:400; kind gift from Raimundo Freire; Hospital Universitario de Canarias, Instituto de Tecnologías Biomédicas, La Laguna, Tenerife, Spain); mouse monoclonal anti-BrdU (clone B44; 1:100; Beckton Dikinson, 347580); rabbit polyclonal anti-GFP (1:1000; Life Technologies, A111-22); mouse monoclonal anti-Vinculin (clone 7F9; 1:5000; Millipore, MAB3574); rabbit polyclonal anti-Dicer1 (1:500; Sigma, HPA00694); rabbit monoclonal anti-Drosha (clone D28B1; 1:1000; Cell Signaling, 3364).

Francia S., Cabrini M., Matti V., Oldani A, & d'Adda di Fagagna F. (2016). DICER, DROSHA and DNA damage response RNAs are necessary for the secondary recruitment of DNA damage response factors. Journal of Cell Science, 129(7), 1468-1476.

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Immunofluorescence Assay for DNA Damage Markers

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Cells were fixed with ice-cold methanol for 15 minutes or 4% paraformaldehyde. Subsequently, cells were permeabilized and blocked with 2% BSA. Primary antibody was added at 1:500 in 1% TBST+1% BSA overnight at 4°C. After 3 washes with 1x PBS, secondary antibody was added at 1:400 with DAPI. After 30 minutes at room temperature, cells were mounted with Prolong Diamond Antifade Mount (Life Technologies). Antibodies used were: 53BP1 (rabbit polyclonal, Novus Biologicals #NB100-304), H2A.X (rabbit polyclonal, Bethyl #A300082A), γ-H2A.X (rabbit polyclonal, phospho-S139; Abcam #ab2893).

Ngo B., Kim E., Osorio-Vasquez V., Doll S., Bustraan S., Liang R.J., Luengo A., Davidson S.M., Ali A., Ferraro G.B., Fischer G.M., Eskandari R., Kang D.S., Ni J., Plasger A., Rajasekhar V.K., Kastenhuber E.R., Bacha S., Sriram R.K., Stein B.D., Bakhoum S.F., Snuderl M., Cotzia P., Healey J.H., Mainolfi N., Suri V., Friedman A., Manfredi M., Sabatini D.M., Jones D.R., Yu M., Zhao J.J., Jain R.K., Keshari K.R., Davies M.A., Vander Heiden M.G., Hernando E., Mann M., Cantley L.C, & Pacold M.E. (2020). Limited Environmental Serine and Glycine Confer Brain Metastasis Sensitivity to PHGDH Inhibition. Cancer discovery, 10(9), 1352-1373.

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