Anti rabbit alexa 594

Anti-MCMV early antigen (EA)²⁷ was used to identify MCMV-infected cells. The preparation and use of FITC-labeled anti-MCMV EA has been described elsewhere.¹³ Rat anti-glycine antibody (specific for amacrine cells) was kindly provided by David V. Pow, University of Queensland, Australia. Mouse anti-IBA1 (specific for macrophages/microglia) was from Wako, Inc. (Richmond, VA, USA). Mouse anti-rhodopsin (specific for photoreceptors) was from Abcam (Cambridge, MA, USA). Mouse anti-GFAP (glial fibrillary acidic protein, specific for glia/Müller cells), FITC-labeled anti-CD11b (specific for macrophages/microglia), FITC-labeled anti-CD3 (specific for CD3⁺ T cells), and FITC-labeled anti-Gr-1 (specific for neutrophils) were all from BD Biosciences (San Jose, CA, USA). Anti-cleaved caspase 3, anti-AIF (apoptosis-inducing factor), anti-caspase 1, anti-poly(ADP-ribose)polymerase 1 (PARP1), anti-nuclear factor–κB (NF-κB), anti-RIP3, goat anti-rabbit IgG-horseradish peroxidase (HRP), and goat anti-mouse IgG-HRP were all from Cell Signaling (Cell Signaling Technology, Inc., Danvers, MA, USA). Anti-β-actin was from Sigma-Aldrich Corp. (St. Louis, MO, USA). Biotin-labeled anti-rat IgG (mouse serum absorbed), anti-mouse Alexa 488, anti-rabbit Alexa 594, AMCA-labeled avidin, FITC-labeled avidin, and Texas red–labeled avidin were from Vector Laboratories, Inc. (Burlingame, CA, USA).