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Psceasy culture medium

Manufactured by Cellapy
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Sourced in China

PSCeasy culture medium is a specialized cell culture medium designed for the maintenance and expansion of human pluripotent stem cells. The medium provides the necessary nutrients and growth factors to support the growth and self-renewal of pluripotent stem cells in culture.

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Lab products found in correlation

Mycoalert plus kit, by Lonza (1 mentions) Matrigel, by Corning (1 mentions)

Spelling variants of this product

PSCeasy medium (11 citations) PSCeasy (2 citations) PSCeasy® II Human Multipotential Stem Cell Culture Medium (1 citations)

2 protocols using psceasy culture medium

1

Maintenance of Human iPSCs

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HiPSCs were purchased from the Cellapy Biological Technology Company. HiPSCs (Cellapy) were routinely maintained in PSCeasy culture medium (Cellapy) and passaged every 3‐4 days using 0.5 mmol/L EDTA (Ethylene Diamine Tetraacetic Acid, Cellapy). Cell lines were used between passages 20 and 85. All cultures were routinely tested for mycoplasma using a MycoAlert Plus Kit (Lonza).
Cui N., Wu F., Lu W., Bai R., Ke B., Liu T., Li L., Lan F, & Cui M. (2019). Doxorubicin‐induced cardiotoxicity is maturation dependent due to the shift from topoisomerase IIα to IIβ in human stem cell derived cardiomyocytes. Journal of Cellular and Molecular Medicine, 23(7), 4627-4639.
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2

hESC-H9 NKX2.5 Reporter Cell Line

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Human embryonic stem cell H9 (hESC-H9) based NKX2.5 reporter cell line (NKX2.5eGFP H9) was purchased from Shanghai model organism Co., Ltd. NKX2.5eGFP H9 and its derived hESC lines were cultured in Matrigel (Corning, USA) coated cell culture plates with PSCeasy culture medium (Cellapy, China) at 37 °C, 5% CO2. When reaching 80–90% confluence, the cells were dissociated with human multipotent stem cell digestive solution (Cellapy), passaged at 1:3 to 1:6, and cultured in PSCeasy medium with ROCK inhibitor Y-27632 (10 μM) for 24 h. Then, the medium was replenished every day without Y-27632.
Guo H., Hang C., Lin B., Lin Z., Xiong H., Zhang M., Lu R., Liu J., Shi D., Xie D., Liu Y., Liang D., Yang J, & Chen Y.H. (2024). HAND factors regulate cardiac lineage commitment and differentiation from human pluripotent stem cells. Stem Cell Research & Therapy, 15, 31.
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