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Sp5 sted cw confocal microscope

Manufactured by Leica
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The SP5 STED-CW confocal microscope is a high-resolution imaging system that combines confocal laser scanning microscopy with continuous-wave stimulated emission depletion (STED-CW) technology. It provides nanoscale resolution for live-cell and fixed-sample imaging.

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Lab products found in correlation

Pluronic f 127, by Thermo Fisher Scientific (1 mentions) Fluo 4 am, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

SP5 confocal microscope (1772 citations) Confocal microscope (1146 citations) SP5 microscope (176 citations) Confocal SP5 (6 citations) SP5-STED microscope (4 citations) STED CW (4 citations) Leica TCS SP5 STED CW confocal microscope (3 citations) SP5-II-STED-CW confocal microscope (2 citations) SP5 II STED-CW Superresolution Laser Scanning Confocal microscope (2 citations)

2 protocols using sp5 sted cw confocal microscope

1

Calcium Imaging with Fluo-4 AM

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Calcium imaging was done using Fluo-4 AM (Invitrogen), which was made into a 50 μM stock solution in Pluronic F-127, which is in 20% dimethyl sulfoxide (Invitrogen). Cell were incubated with 2 μM Fluo-4 AM in media for 30 min and washed after incubation with media. Cells were further incubated for 15 min in media before imaging. Calcium imaging was carried out at a rate of 3.8 Hz with a resolution of 512 × 512 pixels per frame on a Leica SP5 STED-CW confocal microscope in a 37°C incubator.
Nair V., Yi J., Isheim D., Rotenberg M., Meng L., Shi F., Chen X., Gao X., Prominski A., Jiang Y., Yue J., Gallagher C.T., Seidman D.N, & Tian B. (2020). Laser writing of nitrogen-doped silicon carbide for biological modulation. Science Advances, 6(34), eaaz2743.
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2

FRAP Stimulation Experiments on Leica SP5

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Point stimulation experiments were carried out in the FRAP mode of a Leica SP5 STED-CW confocal microscope with a 592-nm laser. Stimulation was carried out for 1 ms and at varying powers.
Nair V., Yi J., Isheim D., Rotenberg M., Meng L., Shi F., Chen X., Gao X., Prominski A., Jiang Y., Yue J., Gallagher C.T., Seidman D.N, & Tian B. (2020). Laser writing of nitrogen-doped silicon carbide for biological modulation. Science Advances, 6(34), eaaz2743.
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