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Anti myc tag

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The Anti-Myc tag is a laboratory reagent used to detect and purify proteins that have been engineered to contain a specific amino acid sequence, known as the Myc tag. The Myc tag is a short peptide that can be added to the protein of interest, allowing it to be identified and isolated using this antibody. The Anti-Myc tag can be used in various techniques, such as Western blotting, immunoprecipitation, and affinity purification, to study the expression, localization, and interactions of Myc-tagged proteins.

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Lab products found in correlation

Vectastain abc hrp kit, by Vector Laboratories (2 mentions) Novared substrate, by Vector Laboratories (2 mentions)

2 protocols using anti myc tag

1

Immunohistochemical Analysis of Oxidized Phospholipids

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Immunohistochemical studies were performed on sections of paraformaldehyde-fixed and paraffin-embedded tissues. Paraffin sections of atherosclerotic lesions, aortic roots and liver tissues were stained for OxPL with biotinylated E06 IgM, or with E06-scFv using a biotinylated mAb anti-Myc tag (Miltenyi Biotech) following the manufacturer’s instruction. Endogenous peroxidase activity was blocked with 0.3% hydrogen peroxide in PBS for 15 min. After blocking, nonspecific binding sites with 10% normal goat serum and Fc Block (2.4G2 antibody) in PBS for 30 min, slides were incubated with primary antibodies for 1 h at room temperature. Biotinylated antibodies (E06, anti-myc and anti-polyHis) were revealed with ABC-HRP VectaStain kit (Vector Laboratories, Burlingame, California) and/or NovaRed substrate (Vector Labs). Slides were counterstained by hematoxylin and in some experiments, percentage of positively stained targets were quantified by image analysis morphometry (Image-Pro Plus).
Que X., Hung M.Y., Yeang C., Gonen A., Prohaska T.A., Sun X., Diehl C., Määttä A., Gaddis D.E., Bowden K., Pattison J., MacDonald J.G., Ylä-Herttuala S., Mellon P.L., Hedrick C.C., Ley K., Miller Y.I., Glass C.K., Peterson K.L., Binder C.J., Tsimikas S, & Witztum J.L. (2018). Oxidized Phospholipids are Proinflammatory and Proatherogenic in Hypercholesterolemic Mice. Nature, 558(7709), 301-306.
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2

Immunohistochemical Analysis of Oxidized Phospholipids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemical studies were performed on sections of paraformaldehyde-fixed and paraffin-embedded tissues. Paraffin sections of atherosclerotic lesions, aortic roots and liver tissues were stained for OxPL with biotinylated E06 IgM, or with E06-scFv using a biotinylated mAb anti-Myc tag (Miltenyi Biotech) following the manufacturer’s instruction. Endogenous peroxidase activity was blocked with 0.3% hydrogen peroxide in PBS for 15 min. After blocking, nonspecific binding sites with 10% normal goat serum and Fc Block (2.4G2 antibody) in PBS for 30 min, slides were incubated with primary antibodies for 1 h at room temperature. Biotinylated antibodies (E06, anti-myc and anti-polyHis) were revealed with ABC-HRP VectaStain kit (Vector Laboratories, Burlingame, California) and/or NovaRed substrate (Vector Labs). Slides were counterstained by hematoxylin and in some experiments, percentage of positively stained targets were quantified by image analysis morphometry (Image-Pro Plus).
Que X., Hung M.Y., Yeang C., Gonen A., Prohaska T.A., Sun X., Diehl C., Määttä A., Gaddis D.E., Bowden K., Pattison J., MacDonald J.G., Ylä-Herttuala S., Mellon P.L., Hedrick C.C., Ley K., Miller Y.I., Glass C.K., Peterson K.L., Binder C.J., Tsimikas S, & Witztum J.L. (2018). Oxidized Phospholipids are Proinflammatory and Proatherogenic in Hypercholesterolemic Mice. Nature, 558(7709), 301-306.
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