The fragments harvested during the grossing of the specimens were routinely processed using a manual technique of histopathologic processing and paraffin embedded. Briefly, 3 μm sections were cut with a semi-automated Rotary Microtome Leica RM2245, on regular slides for routine and special stains, and on precoated slides for immunohistochemical tests. Immunohistochemical (IHC) tests were performed for CD4+ (Helper T lymphocytes), CD8+ (Cytotoxic T lymphocytes) and CD56+ (Natural Killer) cells. Specific details about clones, host, source, dilution and pretreatment are as follows: CD4 (clone 4B12 Leica), mouse, pretreatment with heat-induced epitope retrieval in EDTA, pH 8, dilution 1/200; CD8 (clone 4B11 Leica), mouse, pretreatment with heat-induced epitope retrieval in EDTA, pH 8, dilution 1/100; CD56 (clone CD564 Leica), mouse, pretreatment with heat-induced epitope retrieval in buffer citrate, pH 6, dilution 1/200. The detection system we used was Novolink Polymer (Leica/Novocastra) and DAB chromogen. Immunohistochemical stains were analyzed using an Olympus BX41 microscope.
Cd56 clone cd564
Manufactured by Leica
The CD56 (clone CD564) is a lab equipment product used for the detection and identification of cells expressing the CD56 antigen. It is an antibody-based reagent that can be used in various immunoassay techniques.
Automatically generated - may contain errors
2 protocols using cd56 clone cd564
1
Quantifying Immune Cell Populations
2
Cytopathological Examination of Vitreous Samples
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Cell block preparations were performed as follows. Vitreous perfusion fluid was centrifuged at 3,000 rpm for 3 min and the supernatant was discarded. The dry pellet was fixed in 10% neutral buffered formalin overnight, and then the samples were processed for paraffin embedding and sectioning as usual.
For cytopathological examination, we examined cellularity, background appearance, cell types, and cell atypia. The extents of necrosis, neutrophils, histiocytes, small lymphocytes and atypical lymphocytes were scored as absent (-), mild (+), moderate (++) or marked (+++).
In lymphoma cases or possible lymphoma cases, immunocytochemical staining was performed using antibodies against CD3 (clone LN10, Leica, RTU), CD5 (Clone 4C7, Leica, RTU), CD20 (clone MJ1, Leica, RTU), CD10 (clone 56C6, Leica, RTU), CD56 (clone CD564, Leica, RTU), Bcl-2 (Clone bcl-2/100/D5, Leica, RTU), Bcl-6 (clone LN22, Leica, RTU), MUM-1 9 (clone EAU32, Leica, RTU) and Granzyme B (clone GrB-7, MONOSAN, 1:50). For in situ hybridization, a Bond™ Ready-to-Use ISH EBER Probe (Leica, Catalog No: PB0589probe) was used. 10
For cytopathological examination, we examined cellularity, background appearance, cell types, and cell atypia. The extents of necrosis, neutrophils, histiocytes, small lymphocytes and atypical lymphocytes were scored as absent (-), mild (+), moderate (++) or marked (+++).
In lymphoma cases or possible lymphoma cases, immunocytochemical staining was performed using antibodies against CD3 (clone LN10, Leica, RTU), CD5 (Clone 4C7, Leica, RTU), CD20 (clone MJ1, Leica, RTU), CD10 (clone 56C6, Leica, RTU), CD56 (clone CD564, Leica, RTU), Bcl-2 (Clone bcl-2/100/D5, Leica, RTU), Bcl-6 (clone LN22, Leica, RTU), MUM-1 9 (clone EAU32, Leica, RTU) and Granzyme B (clone GrB-7, MONOSAN, 1:50). For in situ hybridization, a Bond™ Ready-to-Use ISH EBER Probe (Leica, Catalog No: PB0589probe) was used. 10
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