Nb120 19294

Manufactured by Novus Biologicals

Sourced in United States

NB120-19294 is a laboratory equipment product offered by Novus Biologicals. It serves as a tool for researchers and scientists in their scientific investigations and experiments. The core function of this product is to provide a standardized and reliable platform for various laboratory tasks, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Ab137720, by Abcam (1 mentions) Ab7817, by Abcam (1 mentions) Ab56416, by Abcam (1 mentions) Ab9260, by Merck Group (1 mentions) Sc 166736, by Santa Cruz Biotechnology (1 mentions) Dm750, by Leica (1 mentions) Ultravision lp large volume detection system hrp polymer, by Thermo Fisher Scientific (1 mentions) Nb600 408, by Novus Biologicals (1 mentions) Haf008, by R&D Systems (1 mentions)

2 protocols using nb120 19294

Cellular Analysis of Rat Lens Tissue

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For hematoxylin and eosin (H&E) staining and immunohistochemistry, whole rat lenses in our study were fixed in 10% formalin and embedded in paraffin. In immunohistochemistry, the slides were blocked in phosphate-buffered saline (PBS) containing 0.3% Triton X-100 and 3% bovine serum albumin (BSA) for 30 minutes at room temperature (RT) and incubated with primary antibodies at 4°C overnight. After incubation with biotin-conjugated secondary antibodies, the visualization was performed with a DAB staining kit. In immunofluorescence, lenses were fixed in 4% paraformaldehyde (PFA) in PBS and embedded in OCT and cultured LECs were fixed at 4% PFA. After blocking in PBS containing 0.3% Triton X-100 and 3% BSA and incubation with primary antibodies, the cryosections were incubated with fluorescent secondary antibodies. DAPI counterstain was utilized for cell nuclei visualization. Finally, the images were taken by a Leica microscope (DM3000).
The primary antibodies used in immunohistochemistry and immunofluorescence assays were as follows: α-SMA (ab7817; Abcam), FN (ab137720; Abcam), Ki67 (AB9260; Millipore), DNASE 2B (NBP2-16199; Novus), P62 (ab56416; Abcam), TFEB (sc-166736; Santa Cruz), and LAMP1 (NB120-19294; Novus).

Sun Y., Wang X., Chen B., Huang M., Ma P., Xiong L., Huang J., Chen J., Huang S, & Liu Y. (2022). TFEB-Mediated Lysosomal Restoration Alleviates High Glucose-Induced Cataracts Via Attenuating Oxidative Stress. Investigative Ophthalmology & Visual Science, 63(6), 26.

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Histological Analysis of Skin Tissue

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The dorsal skins were fixed with 10% formalin immediately after collection, gradually dehydrated in 80%, 90% and 100% ethanol and cleaned with xylene. Next, the tissues were embedded in paraffin, and hematoxylin and eosin (H&E) staining was carried out. Masson’s trichrome staining was performed for the detection of collagen fibers in the skin tissue. The paraffin-embedded tissues were placed in a 5% iron alum solution for 30 min at 56 °C and then stained with Weigert iron hematoxylin and Biebrich scarlet acid fuchsin solution. After the first stain, the slides were placed in a phosphomolybdic-phosphotungstic acid solution and then stained with aniline blue solution. The stained slides were observed using a light microscope (Leica DM750, Wetzlar, Germany) to detect collagen fibers and nuclei. Immunohistochemistry (IHC) was performed to analyze LAMP-1 and collagen-1 using a UltraVision LP large volume detection system HRP polymer (TL-060-HL, Thermo Scientific) according to the manufacturer’s protocol. LAMP-1/CD107a antibody (1:500, NB120-19294, Novus Biologicals, Centennial, CO, USA) and collagen-1 antibody (1:500, NB600-408, Novus Biologicals) were used as primary antibodies and goat antirabbit IgG secondary antibody (HRP (horseradish peroxidase)) (1:1000, HAF008, R&D Systems, Minneapolis, MN, USA) was used as the secondary antibody.

An M., Kim H., Moon J.M., Ko H.S., Clayton P, & Lim Y.H. (2020). Enzyme-Treated Zizania latifolia Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation. Antioxidants, 9(10), 912.

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