Myiq rt pcr machine

Manufactured by Bio-Rad

The MyIQ RT-PCR machine is a real-time PCR (reverse transcription-polymerase chain reaction) instrument designed for gene expression analysis and quantification. It is capable of performing quantitative, real-time PCR reactions to detect and measure target DNA or RNA sequences.

Automatically generated - may contain errors

Lab products found in correlation

First strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions) Rneasy kit, by Qiagen (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Sybr green supermix, by Bio-Rad (1 mentions) Reverse transcription system, by Promega (1 mentions)

Close spelling variants of this product

PCR machine (73 citations) RT-PCR machine (12 citations) MyIQ machine (7 citations) MyiQ PCR machine (5 citations) MyiQTM (3 citations)

2 protocols using myiq rt pcr machine

Quantitative RT-PCR Influenza Virus Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

At indicated times, total RNA was isolated from whole lung tissue using an RNeasy kit according to the manufacturer's instructions (Qiagen). RNA was then reverse-transcribed into cDNA using the first-strand cDNA synthesis kit according to the manufacturer's instructions (Invitrogen). Viral titre was quantitated by RT-PCR using Genesig (Primer Design) for human influenza virus subtype (H3) following the manufacturer's instructions. β-actin was used to normalize changes in H3 specific gene expression. PCR samples were run and analyzed on a Biorad myIQ RT-PCR machine.

Maroof A., Yorgensen Y.M., Li Y, & Evans J.T. (2014). Intranasal Vaccination Promotes Detrimental Th17-Mediated Immunity against Influenza Infection. PLoS Pathogens, 10(1), e1003875.

+ Open protocol

+ Expand

RT-qPCR Analysis of Inflammatory Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was isolated with Trizol (Life Technologies), treated with DNase, and repurified. cDNA was prepared from 1 µg total RNA, using a reverse transcription system (Promega), and examined by RT-qPCR, using 5 µl of cDNA, 0.3 µM gene-specific primers, and SYBR Green Supermix (Bio-Rad) on a MyIQ RT-PCR machine (Bio-Rad). The following primers were used: human hypoxanthine phosphoribosyl transferase (HPRT): 5’-ACCAGTCAACAGGGGACATAAA AG-3’ (forward); 5’-GTCTGCATTGTTTTGCCAGTGTC-3’ (reverse), TNF-α, forward: 5’-CCCAGGCAGTCAGATCATCTTCC-3’, reverse: 5’-GCTTGAGGGTTTGCTACAACATG-3’; CXCL-8, forward: 5′-CACCGGAAGGAACCATCTCACT-3′, reverse: 5′-TGCACCTTCACACAGAGCTGC-3′; pro-IL-1β: forward: 5’-AAATGATGGCTTATTACAGTGGCA-3’, reverse: 5’-CTTCATCTGTTTAGGGCCATCAG-3’; MKP-1, forward: 5’-CAGTACCCCACTCTACGATCA-3’, reverse: 5’-ACCCTCAAAATGGTTG GGACA-3’; PTP1b, forward: 5’-GCTTCTCCTACCTGGCTGTG-3’; reverse: 5’-TTGTGTGGCTCCAGGATTC-3’; PP2A, forward: 5’-TGCCAATGGTCTCACACTGG-3’; reverse: 5’-GCCTGGTTCCC ACAACGATA-3’; PTPN22, forward: 5’-CCACTTCCTGTACGGACACC-3’, reverse: 5’-TGTTCCACCCCATTCCAGTG-3’. Data were analyzed as described [19 (link),20 (link),26 (link),29 (link)].

Xiong Y., Murphy M., Manavalan T.T., Pattabiraman G., Qiu F., Chang H.H., Ho I.C, & Medvedev A.E. (2015). Endotoxin Tolerance Inhibits Lyn and c-Src Phosphorylation and Association with Toll-Like Receptor 4 but Increases Expression and Activity of Protein Phosphatases. Journal of Innate Immunity, 8(2), 171-184.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!