All chemicals were of analytical grade and were used as received, unless otherwise specified. All aqueous solutions were made with double de-ionised water, 18.2 MΩ cm, with a Pyrogard filter (Millipore, USA). Tris base, MgCl2, NaCl, acetone, NH4OH, HCl, H2O2, 3-glycidoxypropyltrimethoxysilane (GOPTS), PKC-α kinase inhibitor GF 109203X, adenosine tri-phosphate (ATP), adenosine 5'-[γ-thio] triphosphate (ATP-S), 3-mercaptopropyl triethoxysilane (MPTES), 3,3′,5,5′-tetramentylbenzidine (TMB), allyl hydroxyl silane (AHS) and horseradish peroxidase (HRP) were purchased from Sigma-Aldrich. Dephosphorylated myelin basic protein (MBP), purified from bovine brain using FPLC liquid chromatography, was purchased from Millipore. Protein Kinase C alpha (PKC-α) human recombinant kinase produced in Sf9, was procured from ProSec-Tray TechnoGene Ltd. PKC lipid activator cocktail was obtained from Millipore. Polyclonal phospho-(Ser) PKC substrate antibody was purchased from Cell Signaling Technology. 20 nm research grade gold nanoparticles (AuNPs) were bought in colloidal form from Diagnostic Consulting Network.
Pkc lipid activator cocktail
Manufactured by Merck Group
The PKC lipid activator cocktail is a laboratory reagent that contains a mixture of lipids known to activate protein kinase C (PKC), a family of enzymes involved in cellular signaling pathways. This product is designed to be used as a tool for researchers studying PKC-mediated processes in their experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Gf109203x, by Merck Group (2 mentions)
Pyrogard filter, by Merck Group (2 mentions)
Dephosphorylated myelin basic protein, by Merck Group (2 mentions)
Polyclonal phospho ser pkc substrate antibody, by Cell Signaling Technology (2 mentions)
Adenosine 5 γ thio triphosphate, by Merck Group (1 mentions)
3 mercaptopropyl triethoxysilane, by Merck Group (1 mentions)
Horseradish peroxidase hrp, by Merck Group (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
2 protocols using pkc lipid activator cocktail
1
Quantitative Protein Kinase C Activity Assay
2
Characterization of PKC-α Kinase Activity
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All chemicals were of analytical grade and used as received, unless otherwise specified. All aqueous solutions were made with ionised water, 18.2 MΩ cm, with a Pyrogard filter (Millipore, USA). Tris base, magnesium chloride, sodium chloride, acetone, ammonium hydroxide, hydrochloric acid, hydrogen peroxide, mercaptoundecanoic acid (MUA), mercaptohexanol (MCH), ethanolamine, ethanol, NHS (N-hydroxysuccinimide) , EDC (ethyl-dimethylaminopropyl carbodiimide), PKC-α kinase inhibitor GF 109203X, adenosine tri-phosphate (ATP) Gold nanoparticles, 20 nm average spherical diameter, in 0.1 mM PBS were purchased from Sigma-Aldrich. Dephosphorylated myelin basic protein (MBP), purified from bovine brain using fast protein liquid chromatography (FPLC), was purchased from Millipore. PKC-α human recombinant kinase produced in Sf9, was procured from ProSec-Tray TechnoGene Ltd. PKC lipid activator cocktail was obtained from Millipore. Polyclonal phospho-(Ser) PKC substrate antibody was purchased from Cell Signaling Technology.
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