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I bet

Manufactured by Bio-Techne
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Sourced in United Kingdom

I-BET is a compact and versatile lab equipment designed for incubating and mixing biological samples. It provides precise temperature control and gentle agitation to create an optimal environment for cell culture, enzymatic reactions, and other life science applications.

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Lab products found in correlation

Pfi 1, by Bio-Techne (2 mentions) Viktor3, by PerkinElmer (1 mentions) I bet151, by Bio-Techne (1 mentions)

2 protocols using i bet

1

Epigenetic Inhibitor Screening Assay

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Media were replaced with serum-free media containing 0.1% DMSO, 0.1 μM/1 μM/10 μM PFI-1 (Tocris Bioscience, Bristol, UK), 0.1 μM/1 μM/10 μM I-BET (Tocris), or 0.01 μM/0.1 μM/1 μM JQ-1 (Cayman Chemical) compounds. Supernatants were collected 24 h later and assayed by ELISA.
Clifford R.L., Patel J.K., John A.E., Tatler A.L., Mazengarb L., Brightling C.E, & Knox A.J. (2015). CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET. American Journal of Physiology - Lung Cellular and Molecular Physiology, 308(9), L962-L972.
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2

In Vitro Bromodomain Inhibitor Assay

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For in vitro IC50 studies, 5000 cells per well were seeded in 96-well plates, and maintained overnight to allow cell attachment prior to drug exposure. Bromodomain inhibitors used in this study were JQ1 (ApexBio; Cat. No. A1910), i-BET (i-BET 151; Tocris Bioscience; Cat. No. 4650) and PFI-1 (Tocris Bioscience; Cat. No. 4445/10). Cell viability was assessed by the sulforhodamine B (SRB) assay, 72 h after drug treatment. Briefly, cells were fixed with 10% trichloroacetic acid, washed twice with distilled water and stained with 0.4% SRB in 1% acetic acid for 30 min. SRB was then solubilized in 10mM Tris-HCl pH 10.0 and 540 nm-optical densities were determined using a microplate reader (Perkin Elmer Viktor 3). Data was analyzed with GraphPad Prism 5 software.
Ortiz-Barahona V., Soler M., Davalos V., García-Prieto C.A., Janin M., Setien F., Fernández-Rebollo I., Bech-Serra J.J., De La Torre C., Guil S., Villanueva A., Zhang P.H., Yang L., Guarnacci M., Schumann U., Preiss T., Balaseviciute U., Montal R., Llovet J.M, & Esteller M. (2023). Epigenetic inactivation of the 5-methylcytosine RNA methyltransferase NSUN7 is associated with clinical outcome and therapeutic vulnerability in liver cancer. Molecular Cancer, 22, 83.
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