Heraeus labofuge 200

Manufactured by Thermo Fisher Scientific

Sourced in Germany

The Heraeus Labofuge 200 is a compact, high-performance centrifuge designed for general laboratory applications. It features a maximum speed of 6,000 rpm and a maximum RCF of 3,421 x g. The centrifuge can accommodate a range of rotor types and sample volumes.

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Lab products found in correlation

Safeseal micro tube, by Sarstedt (2 mentions) Quantikine human bdnf immunoassay, by R&D Systems (2 mentions)

Spelling variants of this product

Labofuge 200 (6 citations) Heraeus Labofuge 200 centrifuge (2 citations)

3 protocols using heraeus labofuge 200

Oxalic Acid Stripping of Dysprosium

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Equal volumes of an aqueous
oxalic acid solution and the loaded LP phase were contacted for 60
min at room temperature (21 ± 1 °C). The concentration of
oxalic acid used in stripping was the exact stoichiometric amount
needed for precipitation of all rare earths in the LP phase. For a
LP phase containing 0.9 g L^–1 Dy(III), this corresponds
to 8.3 mmol L^–1. After reaching equilibrium, the
phase separation was accelerated by centrifugation (Heraeus Labofuge
200, Thermo Scientific, United States). The remaining Dy(III) concentration
in the LP phase was determined via inductively coupled plasma-optical
emission spectroscopy (ICP-OES), performed with an Avio 500 spectrometer
(PerkinElmer, United States) equipped with a GemCone low-flow nebulizer,
baffled cyclonic spray chamber, alumina injector, and PerkinElmer
Hybrid XLT torch. The samples of the LP phase, the calibration solutions,
the blank solution, and quality controls were diluted in 1-butanol,
with 5 ppm of scandium added as internal standard. The line at 353.170
nm for Dy(III) was measured in axial viewing mode.

Dewulf B., Batchu N.K, & Binnemans K. (2020). Enhanced Separation of Neodymium and Dysprosium by Nonaqueous Solvent Extraction from a Polyethylene Glycol 200 Phase Using the Neutral Extractant Cyanex 923. ACS Sustainable Chemistry & Engineering, 8(51), 19032-19039.

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BDNF Quantification in Serum

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For BDNF analysis, 4.5 mL of venous blood from the antecubital vein was collected with a clotted blood tube at three time points. The first blood sample was taken at the beginning of the experimental session. The second blood sample was collected immediately after the learning phase and the third one was taken immediately after the intervention (exercising or relaxing).
All samples clotted within 30 minutes at a temperature of 21°C. After the clotting period, samples were centrifuged for 10 minutes with 4800 rounds per minute using the Heraeus Labofuge 200 (Thermo Fisher Scientific, Germany). Serum was immediately pipetted into separate SafeSeal microtubes (Sarstedt, Nürnberg, Germany). Samples were stored at −23°C for a mean of 13.5 days and then transferred to a −80°C freezer until the analysis started. BDNF levels in serum were measured using the Quantikine® Human BDNF Immunoassay from R&D Systems (Wiesbaden, Germany) with intra- and interassay coefficients of variation in the range between 8.8 and 11.4 for values between 7.24 and 41.6 ng/mL. The minimum detectable BDNF dose was less than 20 pg/mL, according to the manufacturer's information. BDNF analysis was performed by the Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics in Leipzig.

Hötting K., Schickert N., Kaiser J., Röder B, & Schmidt-Kassow M. (2016). The Effects of Acute Physical Exercise on Memory, Peripheral BDNF, and Cortisol in Young Adults. Neural Plasticity, 2016, 6860573.

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BDNF Quantification in Serum Samples

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In each of the two blood samples, 4.5 ml of venous blood from the antecubital vein were collected with a clotted blood tube. Collection and analysis of blood samples was performed according to the following uniform protocol: all samples clotted within 30 minutes at a temperature of 21C. After the clotting period, samples were centrifuged for 10 minutes with 4800 rounds per minute using the Heraeus Labofuge 200 (Thermo Fisher Scientific, Germany). Immediately afterwards, serum was pipetted into separate SafeSeal micro tubes (Sarstedt, Nürnberg, Germany). Samples were stored at -30°C for one night and then transferred to a -80°C freezer for another three weeks. Then, BDNF levels in serum were measured using the Quantikine® Human BDNF Immunoassay from R&D Systems (Wiesbaden, Germany) with intra- and inter-assay precision of 6.2% and 8.1%, respectively.
The minimum detectable BDNF dose was less than 20 pg/mL, according to the manufacturer’s information. All samples were tested twice for reliability.

Schmidt-Kassow M., Zink N., Mock J., Thiel C., Vogt L., Abel C, & Kaiser J. (2014). Treadmill walking during vocabulary encoding improves verbal long-term memory. Behavioral and Brain Functions : BBF, 10, 24.

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