6–8-wk old WT BALB/c and C57BL/6J female mice were purchased from SPF Biotechnology. Rag1−/− mice were purchased from the Model Animal Research Center of Nanjing University. All mice were maintained under specific pathogen–free conditions.
Female c57bl 6j mice
Manufactured by Spf Biotechnology
Sourced in China
Female C57BL/6J mice are a commonly used mouse strain in biomedical research. They are an inbred strain developed by the Jackson Laboratory. These mice have a black coat color and are widely used as a model organism in various research areas, including immunology, genetics, and neuroscience.
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5 protocols using female c57bl 6j mice
1
Maintenance of Experimental Mouse Models
2
Modeling Psoriasis-like Skin Inflammation in Mice
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C57BL/6J female mice aged 8 weeks were purchased from SPF Biotechnology (Beijing, China). Mice were randomly divided into four groups (n = 6 per group). We intraepidermally injected siKmt2c or control small interfering RNA into the shaved back skin for 3 consecutive days. At the same time, a daily topical dose of 62.5 mg of IMQ (5% Aldara, 3 M Pharmaceuticals, St Paul, MN) cream or Vaseline was used on the back skin of the mice for 6 days. On day 7, all mice were killed. The skin specimens were collected, and then the epidermis was separated for RNA and protein extraction. The siKmt2c sequence is 5'-GCACCACAACTGTTTGATA-3'. The modified psoriasis severity index was based on the PASI: 0, none; 1, mild; 2, moderate; 3, severe; and 4, very severe for erythema, scaling, and thickness. Scores were blindly evaluated by three independent researchers. All animal experiments were approved by the Ethics Committee of Shandong University and conducted according to the National Institutes of Health Guide for the Care and Use of Laboratory Animals.
3
Murine Tumor Immunotherapy Evaluation
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Female C57BL/6J mice (aged 6–8 weeks) were purchased from SPF Biotechnology Co., Ltd. (Beijing, China). For the tumor anti-PD-1 antibody treatment models, mice were subcutaneously implanted with 1 × 106 B16 or LLC cells in the right flank on day 0. A total of 200 μg anti-PD-1 (BioXcell, clone 29 F.1A12) was administered by intraperitoneal injection every 2 days for a total of three times on D7 or D14 after tumor inoculation. Tumor growth was measured, and survival was observed. For the models of the combination of anti-PD-1 antibody and IL-2 treatment, mice were subcutaneously implanted with 1 × 106 B16 cells into the right flank day 0. B16-bearing C57BL/6 mice were left untreated or treated with 200 μg anti-PD-1 (BioXcell, clone 29 F.1A12), IL-2 (1 × 104 U per mouse) therapy, or combination therapy every 2 days for a total of three times, starting from D7 or D14. For OT1+ CD8+ T-cells adoptive transfusion therapy, mice were subcutaneously implanted with 1 × 106 B16 cells into the right flank on day 0. PBS, OT1+ CD8+ T cells overexpression vector, ITGAL, STAT5A, and STAT5B (1 × 106 cells per mouse) were administered via tail vein injection on D7. Tumor growth was measured every second or third day, and tumor volume was calculated as (length×width×width)/2. All animal experiments were approved by the Animal Care and Use Committee of Zhengzhou University.
4
Standardized Mouse Husbandry and Ethics
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Female C57BL/6J mice aged 6 to 8 weeks old and male BABL/c mice aged 6 to 8 weeks old were purchased from SPF Biotechnology Company (Beijing, China). Mice were housed under controlled conditions of temperature (24 °C) with a light/dark cycle of 12/12 h, and with chow and water ad libitum. The experimental protocols used in animal studies were approved by the Institute of Microbiology, University of Chinese Academy of Sciences Ethics Committee. All methods were performed in accordance with the relevant guidelines of the University of Chinese Academy of Sciences.
5
Colitis Induction and Resistant Maltodextrin in Mice
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Female C57BL/6J mice (7 weeks old) were purchased from SPF Biotechnology Co., Ltd. (Beijing, China) and went through a one-week adaptation period. Animals were housed at 22-25°C with a 12 h light/12 h dark cycle. Standard chow and water were provided ad libitum [23 (link)].
All of the mice were randomly divided into four groups (n = 10/group): (i) control group (CON) was provided water; (ii) DSS group was provided 3% DSS (w/v) solution with distilled water; (iii) resistant maltodextrin group (RM) was provided RM (50 mg/kg body weight/day) dissolving in 100 μL PBS by gavage; and (v) RM-DSS group was provided with 3% DSS (w/v) solution with distilled water and RM (50 mg/kg body weight/day) dissolving in 100 μL PBS by gavage. Referring to some published articles [20 (link), 21 (link)], the dose of resistant maltodextrin was chosen based on a pre-experiment, which included two doses (50 mg/kg body weight/day and 100 mg/kg body weight/day). RM was administered for 19 days. On the 14th day of the study, DSS (3% (w/v), molecular weight 36−50 kDa (MP Biomedical, Solon, OH, USA)) was added to drinking water to induce colitis and continued for the next 5 days [23 (link)]. The experimental procedure is shown inFigure 1 .
All of the mice were randomly divided into four groups (n = 10/group): (i) control group (CON) was provided water; (ii) DSS group was provided 3% DSS (w/v) solution with distilled water; (iii) resistant maltodextrin group (RM) was provided RM (50 mg/kg body weight/day) dissolving in 100 μL PBS by gavage; and (v) RM-DSS group was provided with 3% DSS (w/v) solution with distilled water and RM (50 mg/kg body weight/day) dissolving in 100 μL PBS by gavage. Referring to some published articles [20 (link), 21 (link)], the dose of resistant maltodextrin was chosen based on a pre-experiment, which included two doses (50 mg/kg body weight/day and 100 mg/kg body weight/day). RM was administered for 19 days. On the 14th day of the study, DSS (3% (w/v), molecular weight 36−50 kDa (MP Biomedical, Solon, OH, USA)) was added to drinking water to induce colitis and continued for the next 5 days [23 (link)]. The experimental procedure is shown in
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