Normal gastric mucosal cell lines GES-1 and AGS and NCI-N87GC cells were purchased from ATCC (Manassas, VA, USA). The above cells were cultured in RPMI-1640 medium (Gibco, USA) containing 10% FBS (37°C, 5% CO2). ECT2 siRNA (si-ECT2) and si-control (si-NC) were purchased from GenePharma (Shanghai, China). Lipofectamine 2000 (Invitrogen, Carlsbad, USA) was used for cell transfection according to the manufacturer's instructions.
Si control si nc
Manufactured by GenePharma
Sourced in China
Si-control (si-NC) is a laboratory instrument used for the transfection of small interfering RNA (siRNA) into cells. It serves as a negative control to evaluate the efficiency and specificity of siRNA-mediated gene silencing experiments.
Automatically generated - may contain errors
2 protocols using si control si nc
1
Gastric Mucosal Cell Line Culture and Transfection
2
Modulation of circANKS1B and miR-515-5p
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The oligonucleotide sequences of miR-515-5p mimics, miR-con, miR-515-5p inhibitor (anti-miR-515-5p), inhibitor control (anti-NC), overexpression plasmid of circANKS1B (hsa_circ_0007294), si-circANKS1B and si-control (si-NC) were obtained from GenePharma Biotechnology (Shanghai, China). For transfection, cells were seeded into 6-well plates and treated with the above plasmids using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA), as previously described [9 (link)]. The final efficacy of circANKS1B and miR-515-5p expression was analyzed using qRT-PCR.
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