Mouse ifn γ enzyme linked immunosorbent assay elisa kit

Mouse IFN-γ enzyme-linked immunosorbent assay (ELISA) kit was purchased from BD Biosciences, and IL-13 and IL-17 ELISA kits were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). Cytokine levels in the culture supernatants of lymph node and spleen cells were assessed according to the manufacturer’s manual. Briefly, diluted samples were incubated for 2 h with the plate-bound capture antibody. Cytokine expression levels were visualized by horseradish peroxidase (HRP)-conjugated detection antibody in the presence of 3,3′,5,5′-tetramethylbenzidine-1 substrate (BioFX Laboratories, Owings Mills, MD, USA), and absorbance at 450 nm was measured. The anti-TMEV antibody response was determined with ELISA, using UV-TMEV-coated plates, starting with 1/100-diluted serum samples from infected animals as described elsewhere [42 (link)].