Initially, Msalais wine (MW) was centrifuged at 3000× g for 15 min at 4 °C. After that, the supernatant was filtered through a 0.22 μm PTFE membrane filter for analysis.
LC-MS/QTOF analysis was performed on an AB Triple TOF 5600 plus (AB SCIEX, Framingham, MA, USA) mass spectrometer equipped with Waters UPLC (Waters Corp., Milford, MA, USA) and a UV-vis detector. In this process, MS analysis was performed according to the procedure described by Pu [16 (link)], whereas slight modifications were made in the UPLC analysis.
A C18 type column (Agilent ZORBAX, 100 × 4.6 mm, i.d., 1.8 µm) was used to inject 5 μL of the sample at 25 °C, and the flow rate was adjusted to 0.8 mL/min. The mobile phases consisted of 0.1% formic acid (eluent A) and 0.1% formic acid acetonitrile (eluent B). The gradient program was as follows: 0 min, 95% A; 2 min, 95% A; 25 min, 50% A; 35 min, 5% A; 37 min, 5% A; 40 min, 95% A.
LC-MS/QTOF analysis was performed on an AB Triple TOF 5600 plus (AB SCIEX, Framingham, MA, USA) mass spectrometer equipped with Waters UPLC (Waters Corp., Milford, MA, USA) and a UV-vis detector. In this process, MS analysis was performed according to the procedure described by Pu [16 (link)], whereas slight modifications were made in the UPLC analysis.
A C18 type column (Agilent ZORBAX, 100 × 4.6 mm, i.d., 1.8 µm) was used to inject 5 μL of the sample at 25 °C, and the flow rate was adjusted to 0.8 mL/min. The mobile phases consisted of 0.1% formic acid (eluent A) and 0.1% formic acid acetonitrile (eluent B). The gradient program was as follows: 0 min, 95% A; 2 min, 95% A; 25 min, 50% A; 35 min, 5% A; 37 min, 5% A; 40 min, 95% A.