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15n choline chloride

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[15N]-choline chloride is a stable isotope compound used as a research tool in analytical and life science applications. It contains the stable isotope of nitrogen, nitrogen-15, in the choline chloride molecule. The core function of this product is to serve as a tracer or label in studies involving choline metabolism and related biological processes.

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Lab products found in correlation

13c6 d glucose, by Cambridge Isotopes (5 mentions) 15n l tyrosine, by Cambridge Isotopes (5 mentions) 3 3 13c2 cystine, by Cambridge Isotopes (5 mentions) Trimethyl 13c3 caffeine, by Cambridge Isotopes (4 mentions) 15n indole, by Cambridge Isotopes (4 mentions) 2 15n l lysine dihydrochloride, by Cambridge Isotopes (4 mentions) 13c7 benzoic acid, by Cambridge Isotopes (4 mentions) 15n2 uracil, by Cambridge Isotopes (4 mentions) 13c5 l glutamic acid, by Cambridge Isotopes (4 mentions) Formic acid, by Merck Group (3 mentions) 3 4 13c2 cholesterol, by Cambridge Isotopes (3 mentions) 1 2 13c2 palmitic acid, by Cambridge Isotopes (3 mentions) 2 deoxyguanosine 15n2 13c10 5 monophosphate, by Cambridge Isotopes (3 mentions) Lc ms water, by Merck Group (3 mentions) Hplc grade acetonitrile, by Merck Group (3 mentions) Methanol, by Merck Group (1 mentions) Kbro3, by Merck Group (1 mentions) Voso4 h2o, by Merck Group (1 mentions) Milli q, by Merck Group (1 mentions)

5 protocols using 15n choline chloride

1

Isotopic Standards for Mass Spectrometry

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HPLC grade acetonitrile and methanol, LC-MS water and 98% formic acid were obtained from Sigma-Aldrich (St. Louis, MO). A mixture of 14 stable isotopic chemicals were used as internal standards [18 (link)], including [13C6]-D-glucose, [15N]-indole, [2-15N]-L-lysine dihydrochloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [3,4-13C2]-cholesterol, [15N]-L-tyrosine, [trimethyl-13C3]-caffeine, [15N2]-uracil, [3,3-13C2]-cystine, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [15N]-choline chloride and 2’-deoxyguanosine-15N2,13C10-5’-monophosphate from Cambridge Isotope Laboratories, Inc (Andover, PA).
Teeny S., Jarrell Z.R., Krigbaum N.Y., Cirillo P.M., Go Y.M., Cohn B.A, & Jones D.P. (2023). Third Trimester Serum Amino Acid Metabolism is Associated with Maternal Breast Cancer Diagnosed within 15 years of Pregnancy. Research Square.
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2

Isotope-Labeled Internal Standards for Metabolomics

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VOSO4·×H2O and KBrO3 were
purchased from Sigma-Aldrich (St. Louis, MO). Nitric acid was purchased from
MilliporeSigma (Burlington, MA). V2O5 nanoparticles were
purchased from US Research Nanomaterials, Inc. (Houston, TX). An internal
standard mixture for mass spectral analysis consisted of
[13C6]-D-glucose, [15N]-indole,
[2-15N]-L-lysine dihydrochloride,
[13C5]-L-glutamic acid,
[13C7]-benzoic acid,
[3,4-13C2]-cholesterol, [15N]-L-tyrosine,
[trimethyl-13C3]-caffeine,
[15N2]-uracil, [3,3-13C2]-cystine,
[1,2-13C2]-palmitic acid, [15N ,
13C5]-L-methionine, [15N]-choline chloride,
and
2’-deoxyguanosine-15N2,13C10-5’-monophosphate,
all from Cambridge Isotope Laboratories, Inc (Andover, Pennsylvania). Ultra-pure
water (Milli-Q; Sigma-Aldrich, St. Louis, MO) was used as indicated. All
reagents were analytical grade or better unless otherwise stated.
He X., Jarrell Z.R., Ryan Smith M., Thi Ly V., Liang Y., Orr M., Go Y.M, & Jones D.P. (2022). Metabolomics of V2O5 nanoparticles and V2O5 nanofibers in human airway epithelial BEAS-2B cells. Toxicology and applied pharmacology, 459, 116327.
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3

Comprehensive Metabolite Quantification Protocol

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HPLC grade acetonitrile and methanol, LC-MS water and 98% formic acid were obtained from Sigma-Aldrich (St. Louis, MO). A mixture of 14 stable isotopic chemicals were used as an internal standard (Go et al. 2015 (link)) included [13C6]-D-glucose, [15N]-indole, [2-15N]-L-lysine dihydrochloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [3,4-13C2]-cholesterol, [15N]-L-tyrosine, [trimethyl-13C3]-caffeine, [15N2]-uracil, [3,3-13C2]-cystine, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [15N]-choline chloride and 2′-deoxyguanosine-15N132, C10-5′-monophosphate from Cambridge Isotope Laboratories, Inc (Andover, PA).
Go Y.M., Weinberg J., Teeny S., Cirillo P.M., Krigbaum N.Y., Singer G., Tran V., Cohn B.A, & Jones D.P. (2023). Exposome epidemiology for suspect environmental chemical exposures during pregnancy linked to subsequent breast cancer diagnosis. Environment international, 178, 108112.
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4

Targeted Metabolomics Stable Isotope Profiling

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HPLC grade acetonitrile and methanol, LC-MS water and 98% formic acid were obtained from Sigma-Aldrich (St. Louis, MO). A mixture of 14 stable isotopic chemicals used as an internal standard [57 (link)] included [13C6]-D-glucose, [15N]-indole, [2-15N]-L-lysine dihydrochloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [3,4-13C2]-cholesterol, [15N]-L-tyrosine, [trimethyl-13C3]-caffeine, [15N2]-uracil, [3,3-13C2]-cystine, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [15N]-choline chloride and 2’-deoxyguanosine-15N2,13C10-5’-monophosphate from Cambridge Isotope Laboratories, Inc (Andover, PA).
Jarrell Z.R., Smith M.R., Hu X., Orr M., Liu K.H., Quyyumi A.A., Jones D.P, & Go Y.M. (2020). Plasma acylcarnitine levels increase with healthy aging. Aging (Albany NY), 12(13), 13555-13570.
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5

Stable Isotopic Internal Standards for LC-MS

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Unless otherwise stated, materials were purchased from Sigma‐Aldrich. A mixture of 11 stable isotopic chemicals used as an internal standard for LC–MS analysis included [13C6]‐d‐glucose, [15N]‐indole, [15N,13C5]‐l‐methionine, [2‐15N]‐l‐lysine dihydrochloride, [15N]‐choline chloride, [13C5]‐l‐glutamic acid, [13C7]‐benzoic acid, [15N]‐l‐tyrosine, [15N2]‐uracil, [3,3‐13C2]‐cystine and [trimethyl‐13C3]‐caffeine purchased from Cambridge Isotope Laboratories, Inc. PyC2‐Gly [(γEC)2G] standard was purchased from CPC Scientific. All metal standards were purchased from either RICCA Chemical Company or Inorganic Ventures.
Jarrell Z.R., Liu K.H., Dennis K.K., Hu X., Martin G.S., Jones D.P, & Go Y. (2023). Discovery of phytochelatins in human urine: Evidence for function in selenium disposition and protection against cadmium. FASEB BioAdvances, 5(9), 367-375.
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