Small interfering RNAs (siRNAs) were transfected into CCA cell lines using Lipofectamin RNAiMAX reagent (Thermo Fisher Scientific), according to the manufacturer’s instruction. Cells were seeded in a six-well plate at the density of 1.5 × 105 cells/ well (HuCCA-1) and 2 × 105 cells/ well (KKU-213), or in a 96-well plate at the density of 3,000 cells/ well (HuCCA-1) and 4,000 cells/ well (KKU-213) for 24 h prior to transfection with 15 nM siRNA targeting LAMA3 (siLAMA3) (sc-35789; Santa Cruz Biotechnology). AllStars Negative Control siRNA (1027280; Qiagen) was used as control (siNeg). Transfecting medium was replaced with fresh complete media at 24-h post-incubation. Forty-eight hour-post transfection, efficiency of siLAMA3 was evaluated using qRT-PCR and protein expression profile and cell biological phenotypes were assayed as described below.
Sc 35789
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Sc-35789 is a laboratory instrument designed for cell culture applications. It is a fully automated system that performs tasks such as cell seeding, media changes, and sample collection. The product specifications and technical details are available upon request.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sc 35789
1
Knockdown of LAMA3 in Cholangiocarcinoma Cells
2
Integrin β4 and Laminin Receptor Regulation of Cell Migration and Adhesion
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Thirty-six hours prior transfection with 40 nM siRNA targeting integrin β4 (siITGB4) (sc-35678; Santa Cruz Biotechnology, Santa Cruz, CA, USA) using Lipofectamin RNAiMAX reagent (Invitrogen, Carlsbad, CA, USA), HuCCA-1 (2 × 105) cells were seeded in 6-well plate, while KKU-213 (2 × 105) cells were used for transfection with either 20 nM siITGB4 or 25 nM siRNA targeting 37/67 kDa laminin receptor (siLAMR) (sc-35789; Santa Cruz Biotechnology). AllStars Negative Control siRNA (siNeg) (1027280; Qiagen, Valencia, CA, USA) was employed as control. Following 24 hours of transfection, cells were incubated with fresh complete media. Migratory and adhesive abilities of cells induced by laminin were determined at 48-hour post-transfection as described below.
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