Nexera lc 40 system

To synthesize the block copolymers, the sequential RAFT polymerization was performed following an approach modified from Varadhajaran and Delaittre.^[36] The synthetic details are provided in the Supporting Information.
All synthesized compounds were characterized with ¹H nuclear magnetic resonance (NMR) spectroscopy (Bruker Avance III 300, Bruker Avance III 600, or Bruker Avance Neo 700). GPC measurements were performed on a Shimadzu Nexera LC‐40 system (with LC‐40D pump, autosampler SIL‐40C, DGU‐403 (degasser), CBM‐40 (controlling unit), column oven CTO‐40C, UV‐detector SPD40, and RI‐detector RID‐20A).
The system was equipped with 4 analytical GPC‐columns (PSS): 1 × SDV precolumn 3 µm 8×50 mm, 2 × SDV column 3 µm 1000Å 8 × 300 mm, 1 × SDV column 3 µm 10e4Å 8 × 300 mm.
The measurements were performed in THF at a flow speed of 1 mL min⁻¹ at a temperature of 40 °C. Chromatograms were analyzed using the LabSolutions (Shimadzu) software. Calibration was performed against different polymethylmethacrylate standards (800 – 2 200 000 Da, PSS) or polystyrene standards (370 – 2 520 000 Da, PSS).

All extracts were analyzed with a Nexera-LC40 system of Shimadzu Corporation (Kyoto, Japan). This system was equipped with a photodiode array (PDA) detector (SPD-40), a solvent delivery unit (LC-40), an auto-sampler (SIL-40), a column oven (CTO-40), and a system controller SCL-40. All chromatograms were recorded on LabSolutions LC-UV 5.97 SP1 version (Shimadzu Corporation, Kyoto, Japan).
A Cortecs C18 (100 × 3.0 mm) column coupled to a Cortecs C18 VanGuard Cartridge (5 × 3.0 mm), both packed with 2.7 µm superficially porous particles from Waters Corporation (Milford, MA, USA), were used for the analysis of all extracts. The column temperature was maintained at 30 °C. The injection volume was 5 µL. The flow rate was maintained at 1 mL/min. The equilibration time between two injections was 5 min.