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Ab102480

Manufactured by Abcam
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Ab102480 is a recombinant protein that functions as an antibody binding protein. This product is designed for use in various laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

β actin ac 15, by Merck Group (1 mentions) Mouse f4 80 bm8, by Thermo Fisher Scientific (1 mentions) Cleaved caspase 3 d175, by Cell Signaling Technology (1 mentions) Erastin, by Merck Group (1 mentions) B2515, by Merck Group (1 mentions) Shikonin, by Merck Group (1 mentions) L buthionine sulfoximine bso, by Merck Group (1 mentions) Emodin, by Merck Group (1 mentions) S7576, by Merck Group (1 mentions) E7781, by Merck Group (1 mentions) Lsm 700 confocal laser scanning microscope, by Zeiss (1 mentions) Zen 2010, by Zeiss (1 mentions)

2 protocols using ab102480

1

Apoptosis Induction and Analysis Protocol

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The following antibodies were used for Western blotting, confocal microscopy, and flow cytometry analyses: mouse F4/80 (BM8, eBioscience, San Diego, CA, USA), β-actin (AC-15, Sigma-Aldrich), mouse CD74 (05-591, Millipore), human Annexin V (ab14196, Abcam), and cleaved caspase-3 (D175, Cell Signaling). For chemical induction of apoptosis, cells were treated with 10 μM camptothecin at 37°C for 16 h using an apoptosis inducer kit (ab102480; Abcam).
Kim O.H., Kim H., Kang J., Yang D., Kang Y.H., Lee D.H., Cheon G.J., Park S.C, & Oh B.C. (2017). Impaired phagocytosis of apoptotic cells causes accumulation of bone marrow-derived macrophages in aged mice. BMB Reports, 50(1), 43-48.
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2

Apoptosis, Ferroptosis, and Necroptosis Induction

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Apoptosis was induced using camptothecin (10 μM), cycloheximide (200 μM), etoposide (100 μM) (ab102480, Abcam), or anti-FAS antibody (305702, 150 ng/ml, BioLegend). Ferroptosis and necroptosis were induced after treatment with selective activators, Erastin (E7781, 1 μM, Sigma–Aldrich) or L-Buthionine-sulfoximine (BSO) (B2515, 1 μM, Sigma–Aldrich) for ferroptosis and Emodin (E7881, 5 μM, Sigma–Aldrich) or Shikonin (S7576, 1 μM, Sigma–Aldrich) for necroptosis. After fixation, the cells were treated with serum-free protein blocking solution (DAKO) and incubated with anti-PIP3 IgM antibody (Z-P345, 1:100, Echelon Biosciences), CD14, AKT PHD, AKT-PHD/eGFP, or Annexin V. For immunofluorescence studies, tissue sections (2.5 μm) were blocked (DAKO) and incubated with anti-PI(3,4,5)P3 and anti-cleaved caspase 3 antibodies. After mounting, the sections were imaged with an LSM 700 laser-scanning confocal microscope (Carl Zeiss), and images were analyzed with ZEN 2010 Software (Carl Zeiss).
Kim O.H., Kang G.H., Hur J., Lee J., Jung Y., Hong I.S., Lee H., Seo S.Y., Lee D.H., Lee C.S., Lee I.K., Bonner-Weir S., Lee J., Park Y.J., Kim H., Shoelson S.E, & Oh B.C. (2022). Externalized phosphatidylinositides on apoptotic cells are eat-me signals recognized by CD14. Cell Death and Differentiation, 29(7), 1423-1432.
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