L3771

Manufactured by Thermo Fisher Scientific

The L3771 is a laboratory equipment product from Thermo Fisher Scientific. It is designed to perform a core function for laboratory operations, but a detailed description cannot be provided while maintaining an unbiased and factual approach without potential extrapolation.

Automatically generated - may contain errors

Lab products found in correlation

Eclipse ti, by Yokogawa (2 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (2 mentions) Bp337 500, by Thermo Fisher Scientific (2 mentions) Csu x, by Oxford Instruments (2 mentions) Multi port laser unit, by Molecular Devices (2 mentions) Metamorph imaging suite, by Molecular Devices (1 mentions)

2 protocols using l3771

Apoptosis Signaling Pathway Immunostaining

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Cells or tissues were fixed in 4% paraformaldehyde (Electron Microscopy Services, 15710) in 1X PBS for 30 min at room temperature, washed and blocked with a blocking buffer (HBSS fortified with: 10% FBS (Hyclone), 0.1% BSA (Fischer, BP1600), 0.05% saponin (EMD Millipore, L3771), and 0.1% Tween 20 (Fischer, BP337500). Primary antibodies [1:100–1:200] for 2 h at RT or 24 h at 4 °C, Secondary antibodies [1:1000] for 2 h at 22 °C. Samples were imaged with a Nikon Eclipse Ti spinning disc microscope, Yokogawa CSU-X, Andor Zyla sCMOS, Andor Multi-Port Laser unit, and Molecular Devices MetaMorph imaging suite.
Antibodies used: Cleaved Caspase-3 (Asp175) (Cell Signaling, 9661, AB_2341188), HSF1 (Cell Signaling, 4356, AB_2120258), SLC9A1 (sc-136239, AB_2191254), and HSP60 (LK1, sc-59567, AB_783870).

Tharp K.M., Higuchi-Sanabria R., Timblin G.A., Ford B., Garzon-Coral C., Schneider C., Muncie J.M., Stashko C., Daniele J.R., Moore A.S., Frankino P.A., Homentcovschi S., Manoli S.S., Shao H., Richards A.L., Chen K.H., Hoeve J.T., Ku G.M., Hellerstein M., Nomura D.K., Saijo K., Gestwicki J., Dunn A.R., Krogan N.J., Swaney D.L., Dillin A, & Weaver V.M. (2021). Adhesion-mediated mechanosignaling forces mitohormesis. Cell metabolism, 33(7), 1322-1341.e13.

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Immunofluorescence Staining of Cells

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Cells or tissues were fixed in 4% paraformaldehyde (Electron Microscopy Services, 15710) in 1X PBS for 30 min at room temperature, washed and blocked with a blocking buffer (HBSS fortified with: 10% FBS (Hyclone), 0.1% BSA (Fischer, BP1600), 0.05% saponin (EMD, L3771), and 0.1% Tween 20 (Fischer, BP337500). Primary antibodies [1:100-1:200] for 2 h at RT or 24 h at 4 ºC, Secondary antibodies [1:1000] for 2 h at 22 ºC. Samples were imaged with a Nikon Eclipse Ti spinning disc microscope, Yokogawa CSU-X, Andor Zyla sCMOS, Andor Multi-Port Laser unit, and Molecular Devices MetaMorph imaging suite.
Antibodies used: Cleaved Caspase-3 (Asp175) (Cell Signaling, 9661, AB_2341188), HSF1 (Cell Signaling, 4356, AB_2120258), and HSP60 (LK1, sc-59567, AB_783870).

Tharp K.M., Higuchi‐Sanabria R., Timblin G.A., Ford B., Garzón-Coral C., Ward C., Muncie J.M., Stashko C., Daniele J.R., Moore A.S., Frankino P.A., Manoli S.S., Shao H., Richards A., Chen K., Ku G., Hellerstein M.K., Nomura D.K., Saijo K., Gestwicki J.E., Dunn A.R., Krogan N.J., Swaney D.L., Dillin A., & Weaver V.M. (2020). Adhesion-mediated mechanosignaling forces mitohormesis.

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