7890a gc 5975c inert msd

A 1.0 µl of the derivatized extract was injected in splitless mode into a 7890A GC/5975C Inert MSD (Agilent Technologies, Wilmington, DE), coupled with a DB-5 column (30 m*0.25 mm i.d.; film thickness: 0.5 µm; Agilent J&W Scientific, USA). The GC temperature programming was set to 2 min isothermal heating at 80 °C, followed by 10 °C/min oven temperature ramps to 120 °C, 4 °C/min to 260 °C, and then increased at a rate of 10 °C/min to 300 °C, where it was held for 3 min. The inlet temperature was kept at 260 °C and ion source temperature was 200 °C. Helium was used as carrier gas at a constant flow rate of 1 ml/min. Electron impact ionization (70 eV) at full scan mode (m/z 30–600) was used.