The accumulation of H2O2 was detected through the histochemical staining of the leaves with 3,3-diaminobenzidine (DAB) (Sigma-Aldrich St. Louis, MO, USA) method, modified from Daudi and O’Brien (2012) [118 (link)]. Leaves were immersed in DAB solution in the dark on the shaker at 100 rpm for 5 h. The chlorophyll was bleached by incubating the leaves with ethanol at 90 °C for 5 min. The H2O2 concentration was determined as described earlier by Patterson et al. (1984) [119 (link)]. Then, 50 mg of ground leaves of the scions were extracted in 500 µL of precooled acetone and centrifuged for 10 min at 1500× g. Titanium chloride (Sigma-Aldrich St. Louis, MO, USA) (2% v/v of the extracted supernatant) and concentrated ammonia (100 µL) were added to the supernatant prior to centrifuging the reaction mixture at 1500× g for 10 min. Absorbance was read at 410 nm, and the H2O2 concentration was calculated according to the standard curve for peroxide determination.
Titanium chloride
Manufactured by Merck Group
Sourced in United States, Spain
Titanium chloride is an inorganic chemical compound with the formula TiCl4. It is a colorless, fuming liquid at room temperature. Titanium chloride is commonly used as a precursor in the production of titanium metal and titanium dioxide, as well as in various chemical processes and laboratory applications.
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Lab products found in correlation
3 3 diaminobenzidine (dab), by Merck Group (1 mentions)
Cetyl trimethyl ammonium bromide, by ITW Reagents (1 mentions)
Cerium nitrate hexahydrate, by Merck Group (1 mentions)
Ammonia solution, by Merck Group (1 mentions)
Iron 2 chloride, by Merck Group (1 mentions)
Iron 3 chloride hexahydrate, by Merck Group (1 mentions)
Sodium fluoride, by Merck Group (1 mentions)
2 protocols using titanium chloride
1
Quantifying Leaf Hydrogen Peroxide
2
Synthesis of Metal-Oxide Nanoparticles
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Iron (II) chloride (FeCl2, 98 %), iron (III) chloride hexahydrate (FeCl3•6H2O, ≥ 99 %), sodium fluoride (NaF, ≥ 99 %), titanium chloride (TiCl4, ≥ 99.98 %), cerium nitrate hexahydrate (Ce(NO3)3.6H2O, ≥ 99 %), and ammonia solution were purchased from Sigma-Aldrich, Spain. Sodium hydroxide pellets (NaOH), was purchased from Merk.
Hydrochloric acid (HCl), and cetyltrimethylammonium bromide (CTAB) were purchased from Panreac, SA. All the chemicals were of analytical grade or higher, and all solutions were prepared with Milli-Q water and filtered using 0.45 µm Nylon membrane filter.
Hydrochloric acid (HCl), and cetyltrimethylammonium bromide (CTAB) were purchased from Panreac, SA. All the chemicals were of analytical grade or higher, and all solutions were prepared with Milli-Q water and filtered using 0.45 µm Nylon membrane filter.
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