We seeded cells at a density of 2 × 105 cells/well into 6-well plates in RPMI-1640 medium with 10% FBS. After incubation for 24 h, we added various reagents to each well and continued incubation for another 72 h, after which we harvested cells and washed them once with phosphate-buffered saline (PBS). Apoptosis was measured with an Annexin V-Fluorescein Isothiocyanate (FITC)/Propidium Iodide (PI) Cell Apoptosis Detection Kit (TransGen Biotech Co., Ltd., Beijing, China) per manufacturer’s protocols. Cell cycle arrest was measured with a Cell Cycle Staining Kit (Hangzhou Multi-Sciences Biotech Co., Ltd., Hangzhou, China) per manufacturer’s protocols. We performed both analyses using a FACSCalibur using CellQuest software (BD FACS Aria; BD Biosciences, Franklin Lakes, New Jersey, US). All of the experiments were performed at least 3 times.
Annexin 5 fluorescein isothiocyanate fitc propidium iodide cell apoptosis detection kit
Manufactured by Transgene
Sourced in China
The Annexin V-Fluorescein Isothiocyanate (FITC)/Propidium Iodide (PI) Cell Apoptosis Detection Kit is a laboratory tool used to detect and quantify apoptosis, a type of programmed cell death, in cell populations. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and Propidium Iodide, a DNA-binding dye, to distinguish between viable, early apoptotic, and late apoptotic/necrotic cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using annexin 5 fluorescein isothiocyanate fitc propidium iodide cell apoptosis detection kit
1
Apoptosis and Cell Cycle Analysis
2
Quantifying Apoptosis in N2a Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptotic N2a cells were determined using Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) cell apoptosis detection kit (TransGen Biotech Co, Beinjing, China). After treatments, N2a cells were digested with trypsin, washed, and resuspended in Annexin Ⅴ binding buffer. Then, cells were incubated with 5 µL of Annexin V-FITC and 5 µL of PI at room temperature for 15 min in the dark. The number of apoptotic cells were detected using a flow cytometer (FACSVerse, BD Biosciences, Franklin, NJ, USA) and analyzed using FlowJo software (Version 7.6, TreeStar Inc, San Carlos, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!