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P160 rho associated coiled coil kinase rock inhibitor y 27632

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P160-Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 is a small molecule that inhibits the activity of ROCK proteins, which are serine/threonine protein kinases involved in the regulation of cellular processes such as cell adhesion, motility, and contraction.

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Lab products found in correlation

Accutase, by STEMCELL (2 mentions) Growth factor reduced matrigel, by Corning (2 mentions) Transit lti transfection reagent, by Mirus Bio (2 mentions) Mtesr medium, by STEMCELL (2 mentions)

Close spelling variants of this product

Y-27632 (319 citations) ROCK inhibitor Y-27632 (86 citations) ROCK inhibitor (52 citations) Kinase inhibitors (10 citations) Rho-kinase inhibitor Y-27632 (7 citations)

2 protocols using p160 rho associated coiled coil kinase rock inhibitor y 27632

1

Cell Culture and Lentivirus Production

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
RPMI-1640 with 25mM HEPES, 2.0 g/L NaHCO3, 0.3 g/L L-Glutamine supplemented with 10% FBS, 2 mM glutamine, 100 units/mL penicillin and 100 μg/mL streptomycin was used to grow K562 cells. HEK293T cells, used for packaging lentivirus, were grown in Dulbecco’s modified eagle medium (DMEM) in 10% FBS, 100 units/mL penicillin and 100 μg/mL streptomycin. Induced pluripotent stem cells (iPSCs) expressing Cas9 (WTC CRISPRn Gen1C27 (link)) were maintained under feeder-free conditions on growth factor-reduced Matrigel (Corning) in mTeSR medium (STEMCELL Technologies). Accutase (STEMCELL Technologies) was used to enzymatically dissociate iPSCs into single cells to passage with 10 μM p160-Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (Selleckchem) added to promote cell survival. Lentivirus was produced by co-transfecting HEK293T cells with transfer plasmids and standard packaging vectors using TransIT®-LTI Transfection Reagent (Mirus, MIR 2306).
Replogle J.M., Norman T.M., Xu A., Hussmann J.A., Chen J., Cogan J.Z., Meer E.J., Terry J.M., Riordan D.P., Srinivas N., Fiddes I.T., Arthur J.G., Alvarado L.J., Pfeiffer K.A., Mikkelsen T.S., Weissman J.S, & Adamson B. (2020). Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing. Nature biotechnology, 38(8), 954-961.
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2

Cell Culture and Lentivirus Production

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
RPMI-1640 with 25mM HEPES, 2.0 g/L NaHCO3, 0.3 g/L L-Glutamine supplemented with 10% FBS, 2 mM glutamine, 100 units/mL penicillin and 100 μg/mL streptomycin was used to grow K562 cells. HEK293T cells, used for packaging lentivirus, were grown in Dulbecco’s modified eagle medium (DMEM) in 10% FBS, 100 units/mL penicillin and 100 μg/mL streptomycin. Induced pluripotent stem cells (iPSCs) expressing Cas9 (WTC CRISPRn Gen1C27 (link)) were maintained under feeder-free conditions on growth factor-reduced Matrigel (Corning) in mTeSR medium (STEMCELL Technologies). Accutase (STEMCELL Technologies) was used to enzymatically dissociate iPSCs into single cells to passage with 10 μM p160-Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (Selleckchem) added to promote cell survival. Lentivirus was produced by co-transfecting HEK293T cells with transfer plasmids and standard packaging vectors using TransIT®-LTI Transfection Reagent (Mirus, MIR 2306).
Replogle J.M., Norman T.M., Xu A., Hussmann J.A., Chen J., Cogan J.Z., Meer E.J., Terry J.M., Riordan D.P., Srinivas N., Fiddes I.T., Arthur J.G., Alvarado L.J., Pfeiffer K.A., Mikkelsen T.S., Weissman J.S, & Adamson B. (2020). Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing. Nature biotechnology, 38(8), 954-961.
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