Porcine brain l α phosphatidylcholine

The lipids porcine brain L-α-phosphatidylcholine (PC), porcine brain L-α-phosphatidylserine (PS), porcine brain L-α-phosphatidylethanolamine (PE), porcine brain sphingomyelin (SM), bovine liver L-α-phosphatidylinositol (PI), and cholesterol (ovine wool) were purchased from Avanti Polar Lipids (Alabaster, USA). Bovine myelin basic protein 18.5 kDa was purchased from Merck KGaA (Darmstadt, Germany). Buffer solution of N-(2-hydroxyethyl)piperazine-N’-ethanesulfonic acid (HEPES) and sodium chloride (both from Merck KGaA) was prepared with ultrapure water from a Milli-Q Advantage A10 (Millipore S.A.S., Molsheim Cédex, France) with a conductivity lower than 0.055 µS/cm, and it was adjusted with sodium hydroxide (Fisher Scientific, Leicestershire, UK) to pH 7.4. The chloroform used had HPLC grade and was purchased from Carl Roth GmbH & Co. KG (Karlsruhe, Germany). The fluorescent dye 1,2-Dihexadecanoyl-sn-glycero-3-phosphoethanol-amine-N-(lissamine rhodamine B sulfonyl) (Rh−DHPE) was obtained from Life Technologies GmbH (Darmstadt, Germany) and TopFluor^® Cholesterol was obtained from Avanti Polar Lipids (Alabaster, USA). All chemicals were used as received without further purification.