4 hydroxynonenal hne adduct assay

We purchased the reagents for the measurement of ethanol from Analox instruments (London, UK). 4-Hydroxynonenal (HNE) adduct assay was from Cell Biolabs, Inc. (San Diego, CA). Rapamycin was from EMD Millipore (Burlington, MA). ATF6 was from LifeSpan Biosciences (Seattle, WA). mTOR, p-mTOR, 4EBP1, p- 4EBP1, p70S6K, p-p70S6K, eukaryotic initiation factor 2α (eIF2α), p-eIF2α, Ki-67, p- PERK, cleaved caspase-3, and cleaved caspase-12 antibodies, were from Cell Signaling Technology (Danvers, MA). GRP78 antibody was from Novus Biologicals (Littleton, CO). X-box binding protein-1s (XBP1s) antibody was from BioLegend (San Diego, CA). Doublecortin (DCX) antibody was from Abcam (Cambridge, MA). CCAAT/- enhancer-binding protein homologous protein (CHOP) antibody was from Thermo Fisher Scientific (Rockford, IL). HRP-conjugated anti-rabbit and anti-mouse secondary antibodies were from GE Healthcare Life Sciences (Piscataway, NJ). Mounting media containing 4’, 6-diamidino-2-phenylindole (DAPI) was from Vector Laboratories (Burlingame, CA). Alexa-488 conjugated anti-rabbit and Alexa-594 conjugated anti mouse antibodies were from Life Technologies (Grand Island, NY). Ketamine/xylazine was from Butler Schein Animal Health (Dublin, OH). Other chemicals and reagents were purchased either from Sigma-Aldrich or Life Technologies (Frederick, MD).

Anti-GRP78, anti-eIF2a, anti-DNP, and anti-ATF6 antibodies were obtained from Santa Cruz Biotech (Santa Cruz, CA). Anti-cleaved caspase-3, anti-phospho-PERK, and anti-IRE1a antibodies were obtained from Cell Signaling Technology, Inc. (Beverly, MA). Anti-phospho-IRE1a (Ser724), anti-MANF, and anti-caspase-12 antibodies were obtained from Abcam (Cambridge, MA). Anti-X-box binding protein-1s (XBP1s) antibody was from BioLegend (San Diego, CA). 4-PBA and NAC were purchased from Sigma Chemical Co. (St. Louis, MO). 4-Hydroxynonenal (HNE) adduct assay was obtained from Cell Biolabs, Inc. (San Diego, CA). Small interfering (si) RNA for human MANF gene (si MANF) was purchased from invitrogen Co. (Carlsbad, CA). TUNEL Assay Kit was obtained from Abcam (Cambridge, MA). STF-083010 was obtained from Med Chem express (New Jersey, USA). Salubrinal was purchased from Tocris Bioscience (Avonmouth, Bristol, UK). Recombinant human MANF was obtained from Creative BioMart company (Shirley, NY). All other antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Other chemicals/reagents used in this project were obtained from Thermo Fisher Scientific, Inc. (Waltham, MA, USA) unless stated otherwise.