The peptide sequences derived from the gp41 MPER-TMD region displayed in Figure 1b were produced as C-terminal carboxamides by solid-phase synthesis using Fmoc chemistry and purified by HPLC. PC-based synthetic phospholipids and dodecylphosphocholine (DPC) were purchased from Avanti Polar Lipids (Birmingham, AL, USA). N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (NBD-PE) was from Thermo Fisher Scientific (Waltham, Massachusetts, USA). Abberior STAR RED (KK114) was obtained from Abberior (Göttingen, Germany). 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) was obtained from Sigma-Aldrich (St. Louis, Missouri, USA).
Star red kk114
Manufactured by Abberior
Sourced in Germany
Abberior STAR RED (KK114) is a fluorescent label designed for use in fluorescence microscopy. It is a small, bright, and photostable dye that can be used to label biomolecules, such as proteins, DNA, or small molecules. The dye has an excitation maximum at 637 nm and an emission maximum at 665 nm, making it suitable for detection in the red region of the spectrum.
Automatically generated - may contain errors
Lab products found in correlation
1 1 1 3 3 3 hexafluoro 2 propanol hfip, by Merck Group (1 mentions)
N 7 nitrobenz 2 oxa 1 3 diazol 4 yl 1 2 dihexadecanoyl sn glycero 3 phosphoethanolamine nbd pe, by Thermo Fisher Scientific (1 mentions)
Dodecylphosphocholine dpc, by Avanti Polar Lipids (1 mentions)
Star 635p, by Abberior (1 mentions)
2 protocols using star red kk114
1
Peptide Synthesis and Lipid Preparation
2
Fluorescent Labeling of Antibody Fragments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human anti-gp120 monoclonal antibody 2G12 and b12 were purchased from Polymun Scientific, and mouse anti-MHC-I monoclonal antibody W6/32 was produced in house from hybridoma stocks37 (link). Fab fragments were generated using the Fab Micro Preparation kit (Pierce) according to the manufacturer’s instructions. Anti-human and anti-mouse IgG Fab fragments (Jackson ImmunoResearch) were coupled to Abberior STAR 635P or Abberior STAR RED (KK114) dyes (Abberior GmbH, Göttingen, Germany) via NHS-ester chemistry according to the dye manufacturer’s instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!