Huperzine a

The following reagents and standard substances were used in the tests: Acetonitrile HPLC grade, Methanol HPLC grade Chromasolv^®, Sodium hexafluorophosphate (NaPF₆) and Ammonium acetate. Cellulase and Pectinase were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). HPLC grade water was purchased from Merck KGaA (Darmstad, Germany). LC-MS H₂O (18 Ω) grade was purchased from Millipore Merck KGaA (Darmstadt, Germany). LC-MS acetonitrile grade was purchased from Merck. Chloroform, diethyl ether, sodium chloride, ethyl alcohol, formaldehyde 37% and glacial acetic were purchased from POCH (Gliwice, Poland). Bacto™ Agar was purchased from Becton, Dickinson & Company, (Franklin Lakes, New Jersey, USA). IAA, IBA and kinetin were purchased from Grand Island Biological Company (New York, USA). Plant Preservative Mixture (PPM) was purchased from Plant Cell Technology (Washington, D.C., USA). Toluidine Blue was purchased from Across Organics (Geel, Belgium). Periodic acid and Schiff’s reagent for microscopy was purchased from Merck KGaA. Huperzine A and Huperzine B standards were purchased from ChromaDex Inc. (Laguna Hills, CA, USA).