Hsp60 h 1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Hsp60 (H-1) is a heat shock protein that functions as a molecular chaperone, assisting in the folding and assembly of proteins within cells. This product is suitable for research applications.

Automatically generated - may contain errors

Lab products found in correlation

Relb cflag pcdna3, by Addgene (1 mentions) P52 cflag pcdna3, by Addgene (1 mentions) C rel cflag pcdna3, by Addgene (1 mentions) γ xmg1.2, by Thermo Fisher Scientific (1 mentions)

2 protocols using hsp60 h 1

Generating NF-κB Signaling Plasmids and Antibodies

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pMIGR1-mP52 and pMIGR1-mGM-CSF were generated by inserting mouse p52 (encoding amino acids 1–405 of NF-κB2) and mouse Csf2 cDNAs, respectively, into the EcoRI and BglII sites of pMIGR1-GFP vector. pcDNA-based expression vectors encoding FLAG-tagged mouse p52 (p52-cFlag-pcDNA3), mouse c-Rel (c-Rel-cFlag-pcDNA3), and mouse RelB (RelB-cFlag-pcDNA3) were from Addgene. The Csf2 promoter luciferase plasmid (pGL3-mCsf2), covering the region −225 to +26 of mouse Csf2 promoter (15 (link)), was provided by Dr. Takeshi Matsumura (Kumamoto University).
Functional grade anti–mouse (m) CD3ɛ (145-2C11) and anti-mCD28 (37.51) antibodies and blocking antibodies for mIFN-γ (XMG1.2) and mIL-4 (11B11) were from eBioscience. Fluorescence-labeled antibodies for mCD4 (L3T4), mCD8 (53-6.7), mCD3 (145-2C11), CD44 (IM7), CD62L (MEL-14), IL-17A (eBio17B7), GM-CSF (MP1-22E9), and IFN-γ (XMG1.2) were purchased from eBioscience. Antibodies for mouse p50 (C-19), c-Rel (sc-70), RelB (C-19), NIK (H-248), Lamin B (C-20), and Hsp60 (H-1) were from Santa Cruz Biotechnology. An antibody recognizing both p100 and p52 (Anti-p52/p100) was provided by NCI Preclinical Repository.

Yu J., Zhou X., Nakaya M., Jin W., Cheng X, & Sun S.C. (2014). T Cell Intrinsic Function of the Noncanonical NF-κB Pathway in the Regulation of GM-CSF Expression and EAE Pathogenesis. Journal of immunology (Baltimore, Md. : 1950), 193(1), 422-430.

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Immunoblotting Procedures with Various Antibodies

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PC2 (H-280), GFP (B-2), FLNA (E-3), FLNA (H-300), β-actin (C4), Na/K ATPase (H-300) and HSP60 (H-1) antibodies were purchased from Santa Cruz Biotech (Santa Cruz, Dallas, TX, USA). PC2 (1A11) mouse polyclonal antibody [26 (link),29 (link)] and PC2 (H-280) was used to detect native PC2. Antibody E-3 against the N-terminus of FLNA was used to detect full-length FLNA while antibody H-300 was used to detect both the full length and C-terminus of FLNA (FLNAC, aa 2150–2647). Goat anti-GFP (EU4) was purchased from Eusera (Eusera, Edmonton, AB, Canada). Secondary antibodies were purchased from GE Healthcare (Baie d’Urfe, QC, Canada).

Wang Q., Zheng W., Wang Z., Yang J., Hussein S., Tang J, & Chen X.Z. (2015). Filamin-A Increases the Stability and Plasma Membrane Expression of Polycystin-2. PLoS ONE, 10(4), e0123018.

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