Mercury plus 400 mhz spectrometer

NMR spectra were recorded on Varian Mercury Plus 400 MHz spectrometer at 400 MHz for ¹H NMR and 100 MHz for ¹³C NMR. ¹³C and ¹H NMR spectra were taken using acetone-d6 (for 4-((3-benzylureido)methyl)furan-3-carbonyl azide) (3) and DMSO-d6 (for DHFP) as a solvent. Infrared spectra were recorded on Perkin Elmer FTIR/FIR Spectrophotometer Frontier. All high-resolution mass spectra (HRMS) were measured on UPLC-UHR-Q/TOFABSCIEX Triple TOF 4600. Uncorrected melting points were determined on the Electrothermal melting point analyzer.
4-(isocyanatomethyl)furan-3-carbonyl azide was used as starting material and was synthesized according to the Yılmaz et al. procedure [23 (link)]. The chemicals used in the experiments were obtained from commercial sources. All reactions were monitored by TLC (Thin Layer Chromatography) with Merck 0.2 mm silica gel 60 F254 aluminum plates, and silica gel (Merck 70-230 mesh) was used in column chromatography.

¹H and ¹³C nuclear magnetic resonance (NMR) spectra were acquired on a Varian Mercury Plus 400 MHz spectrometer at 20°C. Molecular weights and molecular weight distributions were determined by gel permeation chromatography (GPC) using PE series 200 with RI-WAT 150CV+ as detector, THF as the mobile phase at a flow rate of 1 mL/min and polyethylene glycol (PEG) as a standard at 70°C. Light scattering data were recorded through Viscotek/Malvern GPC system consisting of a GPC Max autoinjector fitted to a TDA 305 triple detector array (differential RI, right angle light scattering (RALS), low angle static light scattering (LALS), and four-capillary differential viscometer detectors) using LiBr/DMF (0.02 mol/L) as eluent at a flow rate of 0.7 mL/min and linear poly(methyl methacrylate) as calibration at 50°C. Fourier transform infrared (FTIR) spectra were recorded on a PE Paragon 1000 spectrometer (KBr disk).