The RBC ghost moiety (i.e., porous, lysed cells) not used for enzyme assay was directly lipid-extracted according to the method of Folch et al. (1957) [48 (link)] and the gained complex lipids’ (FA) composition was determined with gas chromatography (Shimadzu Nexis 2030, Kyoto, Japan), in the form of fatty acid methyl esters [36 (link)], after a separation on a Phenomenex Zebron ZB-Wax capillary column (30 m × 0.25 mm × 0.25 micrometer film, Phenomenex Inc., Torrance, CA, USA). The chromatographic evaluation was performed with the LabSolutions 5.93 software, using the PostRun module (Shimadzu, Kyoto, Japan) with manual peak integration. Fatty acid composition was expressed as weight % of total FA methyl esters. The identification of the FAs was performed based on the retention time of a CRM external standard (Supelco 37 Component FAME Mix, Merck-Sigma Aldrich, CRM47885).
Nexis 2030
Manufactured by Shimadzu
Sourced in Japan
The Nexis 2030 is a compact and versatile gas chromatograph (GC) system designed for a wide range of analytical applications. It features a robust and reliable design, providing consistent performance and accurate results. The Nexis 2030 GC system is capable of handling a variety of sample types and can be configured with different detectors to meet the specific needs of your laboratory.
Automatically generated - may contain errors
Lab products found in correlation
Supelco 37 component fame mix, by Merck Group (1 mentions)
Zebron zb wax capillary column, by Phenomenex (1 mentions)
Zorbax eclipse plus c18 column, by Agilent Technologies (1 mentions)
Aoc 6000, by Shimadzu (1 mentions)
Masshunter workstation b 07, by Agilent Technologies (1 mentions)
6495 triple quadrupole, by Agilent Technologies (1 mentions)
Tq8040 nx, by Shimadzu (1 mentions)
Aquity uplc, by Waters Corporation (1 mentions)
6 protocols using nexis 2030
1
Extraction and Analysis of RBC Lipids
2
Hydrogenation of Furfural with Catalyst
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In a typical reaction, furfural and catalyst were put into a 25 mL NSC-type autoclave (Anhui CHEMN Instruments Co., Ltd.) with 10 mL of pure water as solvent. After being purged for 3 times with H2, the reactor was charged with H2 to the set pressure. Then, the reactor was heated up to reaction temperature with 10 °C/min ramping rate and kept for a certain time. After reaction, the reactor was cooled down to room temperature and n-hexanol was added as internal standard. The liquid product was analyzed by Shimadzu Nexis 2030 (Kyoto, Japan) gas chromatography (GC). The solid residue was washed with pure water for the next-run usage.
3
Comprehensive GC-MS/MS and UHPLC-MS/MS Analysis
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A Shimadzu Nexis 2030 gas chromatograph equipped with a programmed split/splitless injector and an AOC 6000 multifunction autosampler (Shimadzu, Kyoto, Japan), and a Shimadzu 8040 138 NX tandem mass spectrometry (Shimadzu, Kyoto, Japan) were used to perform gas chromatography coupled with tandem mass spectrometry (GC-MS/MS) confirmation. An SH-Rxi-5Sil MS (30 m 140 × 0.25 mm id × 0.25 μm film) capillary column was used. A 1290 Infinity UHPLC system was linked to a 6495 Triple Quadrupole LC-MS/MS device added with a jet stream EI source (Agilent, Santa Clara, CA, USA). Data were acquired and analyzed on an Agilent MassHunter Workstation B.07.00. Chromatographic isolation was finished on an Agilent ZORBAX Eclipse Plus C18 column (50 mm × 2.1 mm, 1.8 μm) with gradient elution.
Samples were prepared using a GENIUS 3 vortex agitator (IKA, Stauffen, Germany), a CL31R multispeed refrigerated centrifuge (Thermo Scientific, Waltham, MA, USA), a WD12 water bath nitrogen blowing instrument (Aosheng Instrument, Hangzhou, China), and a CK2000 high-throughput tissue grinder (Thmorgan Biotechnology, Beijing, China).
Samples were prepared using a GENIUS 3 vortex agitator (IKA, Stauffen, Germany), a CL31R multispeed refrigerated centrifuge (Thermo Scientific, Waltham, MA, USA), a WD12 water bath nitrogen blowing instrument (Aosheng Instrument, Hangzhou, China), and a CK2000 high-throughput tissue grinder (Thmorgan Biotechnology, Beijing, China).
4
Photoelectrochemical Hydrogen Evolution
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Measurements
of the evolution of hydrogen gas were made in a two-compartment electrochemical
cell setup made of glass under one sun’s light using a solar
light simulator (Thermo Oriel 92194-1000) fitted with a Newport AM
1.5G filter. The prepared WO3/BiVO4/sprayed
TiO2/sputtered TiO2 and WO3/BiVO4/sprayed TiO2/sputtered TiO2/NiOOH electrodes
were the photoanodes employed in the anode compartment. The reference
electrode was an Ag/AgCl electrode, whereas the counterelectrode was
a platinum (Pt) mesh. We employed 0.5 M aqueous Na2SO4 as the electrolyte. A Nafion membrane divided the anode and
cathode compartments, and a rubber stopper effectively sealed the
cathode compartment. Nitrogen gas (N2) was continuously
purged from the sample headspace at a rate of 10 mL min–1. Gas chromatography (GC) (Shimadzu Nexis 2030) was used to track
the evolution of hydrogen (H2), and an autosampler was
set up to continuously inject 2 mL of the headspace stream into the
system. Prior to injection, the gas samples were passed through a
2 mL sample loop (Restek). The measured H2 content in the
purge gas and the purge gas flow rate were used to compute the hydrogen
evolution rates. A comprehensive protocol for quantifying hydrogen
is outlined in our previous reports.39 (link),40 (link)
of the evolution of hydrogen gas were made in a two-compartment electrochemical
cell setup made of glass under one sun’s light using a solar
light simulator (Thermo Oriel 92194-1000) fitted with a Newport AM
1.5G filter. The prepared WO3/BiVO4/sprayed
TiO2/sputtered TiO2 and WO3/BiVO4/sprayed TiO2/sputtered TiO2/NiOOH electrodes
were the photoanodes employed in the anode compartment. The reference
electrode was an Ag/AgCl electrode, whereas the counterelectrode was
a platinum (Pt) mesh. We employed 0.5 M aqueous Na2SO4 as the electrolyte. A Nafion membrane divided the anode and
cathode compartments, and a rubber stopper effectively sealed the
cathode compartment. Nitrogen gas (N2) was continuously
purged from the sample headspace at a rate of 10 mL min–1. Gas chromatography (GC) (Shimadzu Nexis 2030) was used to track
the evolution of hydrogen (H2), and an autosampler was
set up to continuously inject 2 mL of the headspace stream into the
system. Prior to injection, the gas samples were passed through a
2 mL sample loop (Restek). The measured H2 content in the
purge gas and the purge gas flow rate were used to compute the hydrogen
evolution rates. A comprehensive protocol for quantifying hydrogen
is outlined in our previous reports.39 (link),40 (link)
5
Extraction and Analysis of Pesticide Residues in Apples
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Extraction and cleanup of unpeeled whole apples were done as recommended by pesticide residue analysis manual 2007, ICAR (38 ). Briefly, gas chromatograph (Nexis 2030, Shimadzu, Japan) with mass selective triple quadruple detector (GC-MS/MS TQ 8040 NX, M/s Shimadzu, Japan) and liquid chromatography (Aquity UPLC, Waters Corporation, USA) fitted with mass detector (AB 3200, AB Sciex, USA) was used for analysis. For separation of analytes through GC-MS and LC-MS/MS, SH-Rxi-5Sil MS and Chromolith RP–18 columns were used, respectively (39 (link)).
6
Fatty Acid Profiling by GC-FID
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Brain lipids were extracted by two commonly used solvents, chloroform, and methanol, in a volume ratio of 2:1. The methyl ester fatty acids were derived from a BH3/methanol reagent and analyzed using Shimadzu Nexis 2030 gas chromatography with a detection system of flame-ionization. The chromatography column was Dikmacap-2330 (60 m×0.25 mm). Fatty acid separation was acquired in a two-stage temperature program by maintaining the initial temperature at 60°C for 2 minutes and increasing it to 200°C at a rate of 10°C/min for 25 minutes. Then column reaches the final temperature of 240°C at a rate of 5°C/min and keeps it for 7 minutes. The chromatogram was analyzed by comparing the retention times of each peak with the peaks from the standard fatty acid methyl esters. Data were presented as the relative percentage by calculating the peak area ratio for each fatty acid to the total area from the rest of the peaks by the LabSolutions software.
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