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Human igg isotype control

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The Human IgG isotype control is a reagent used in immunoassays to establish the specificity of antibody binding. It serves as a negative control, providing a baseline for comparison to assess the specificity of the target antibody.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (4 mentions) Neratinib, by Selleck Chemicals (2 mentions) Capecitabine, by Selleck Chemicals (2 mentions) Crizotinib, by Selleck Chemicals (2 mentions) Lapatinib, by Selleck Chemicals (2 mentions) Trametinib, by Selleck Chemicals (2 mentions) Goat anti human igg hrp, by Abcam (1 mentions)

3 protocols using human igg isotype control

1

Quantitative Fluorescent Imaging of Drug Combinations

Cited 1 time
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Lapatinib, neratinib, crizotinib, trametinib, capecitabine (Selleckchem), pertuzumab, and trastuzumab (OHSU Pharmacy) were used at the concentrations indicated in figure legends. DMSO (ThermoFisher) and human IgG isotype control (Abcam) concentrations were equivalent to the highest dose of the respective drug used in each experiment. Treatment durations were as indicated in respective figure legends. Cells were treated in 96 and 384 multiwell plates with soluble drug and ligand combinations added to their medium, then fixed for fluorescent imaging and quantification (described below). Each treated cell line was seeded at an experimentally determined concertation so that untreated control wells would reach 80% confluency by the end of the treatment period. Drug combination studies and CTG assays in Figure 4F were performed as previously reported (Heiser et al., 2012 (link)) (Kuo et al., 2009 (link)) in randomized replicates.
Watson S.S., Dane M., Chin K., Tatarova Z., Liu M., Liby T., Thompson W., Smith R., Nederlof M., Bucher E., Kilburn D., Whitman M., Sudar D., Mills G.B., Heiser L.M., Jonas O., Gray J.W, & Korkola J.E. (2018). Microenvironment-Mediated Mechanisms of Resistance to HER2 Inhibitors Differ between HER2+ Breast Cancer Subtypes. Cell systems, 6(3), 329-342.e6.
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2

Quantitative Fluorescent Imaging of Drug Combinations

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Lapatinib, neratinib, crizotinib, trametinib, capecitabine (Selleckchem), pertuzumab, and trastuzumab (OHSU Pharmacy) were used at the concentrations indicated in figure legends. DMSO (ThermoFisher) and human IgG isotype control (Abcam) concentrations were equivalent to the highest dose of the respective drug used in each experiment. Treatment durations were as indicated in respective figure legends. Cells were treated in 96 and 384 multiwell plates with soluble drug and ligand combinations added to their medium, then fixed for fluorescent imaging and quantification (described below). Each treated cell line was seeded at an experimentally determined concertation so that untreated control wells would reach 80% confluency by the end of the treatment period. Drug combination studies and CTG assays in Figure 4F were performed as previously reported (Heiser et al., 2012 (link)) (Kuo et al., 2009 (link)) in randomized replicates.
Watson S.S., Dane M., Chin K., Tatarova Z., Liu M., Liby T., Thompson W., Smith R., Nederlof M., Bucher E., Kilburn D., Whitman M., Sudar D., Mills G.B., Heiser L.M., Jonas O., Gray J.W, & Korkola J.E. (2018). Microenvironment-Mediated Mechanisms of Resistance to HER2 Inhibitors Differ between HER2+ Breast Cancer Subtypes. Cell systems, 6(3), 329-342.e6.
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3

Quantitative ELISA for Total IgG

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Total IgG was measured using ELISA as described above, except that dilution standards were used to coat plates with either bicarbonate/carbonate-coating buffer only or with a 1:5000 dilution of bicarbonate/carbonate coating buffer and patient sera. Dilution standards were prepared using Human IgG Isotype Control (Abcam). All dilutions performed in duplicate. Patient dilutions were performed using 2-fold serial dilutions in triplicate starting at a 1:100,000 dilution with each well containing 50μg diluted sera. Goat anti-human IgG-HRP (Abcam) was diluted to 1:1000 and measured using the above protocol.
Milner C.A., Alteri C.J, & Mobley H.L. (2019). UTI patients have pre-existing antigen-specific antibody titers against UTI vaccine antigens. Vaccine, 37(35), 4937-4946.
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