Lysozyme cre

VLDLR^−/− mice^{71 (link)}, Reln^−/− mice^{72 (link)}, Tsc1 flox mice^{73 (link)}, Raptor flox mice^{74 (link)}, Lysozyme-Cre^{75 (link)}, and Vav1-iCre transgenic mice^{43 (link)} were from Jackson Laboratory and maintained on C57BL/6 background. Mice were fed standard rodent chow ad libitum (Harlan Laboratories). To generate VLDLR^−/− and wildtype littermates, VLDLR^+/− female were bred with VLDLR^+/− male mice. To generate Reln^−/− and wild-type littermates, Reln^+/− female were bred with Reln^+/− male mice. To obtain Raptor^flox/flox;Vav1-iCre (or TSC1^flox/flox;Vav1-iCre) cKO mice, male Raptor^flox/flox (or TSC1^flox/flox) mice were bred with Raptor^flox/+;Vav1-iCre (or TSC1^flox/+;Vav1-iCre) female mice^{76 (link)}. To obtain Raptor^flox/flox;Lysozyme-Cre (or TSC1^flox/flox;Lysozyme-Cre) cKO mice, female Raptor^flox/flox (or TSC1^flox/flox) mice were bred with Raptor^flox/flox;Lysozyme-Cre (or TSC1^flox/flox;Lysozyme-Cre) male mice. All experiments were conducted using littermates. Sample size estimate was based on power analyses performed using SAS 9.3 TS X64_7PRO platform. All animal experiments were approved by the Institutional Animal Care and Use Committee of UT Southwestern Medical Center.

To generate deletion of Trim33 in mature myeloid cells, Trim33^fl/fl C57Bl/6-CD45.2 mice15 (link) were crossed with Lysozyme-Cre C57Bl/6-CD45.2 mice (strain name: B6.129P2-Lyz2t^m1(cre)Ifo/J, The Jackson Laboratory). Male and female mice of 8–12 months of age were used. Experiments were performed in compliance with European legislation and with the Ethics Committee of the French Ministry of Agriculture (Agreement B9203202).