Observer axio vision a 1

Nine thousand P. gallinaceum mature sporozoites were pulled from the salivary glands of A. aegypti and incubated in 50 μL of PBS solution, with 40 μmol L^–1 digitonin (positive control), 60 μmol L^–1 peptides or negative control (PBS solution), at 37 °C for one hour. Cell membrane integrity was then observed using a Carl Zeiss inverted fluorescence microscope (model Observer Axio Vision A.1) coupled to an image capture Zeiss AxioCam HR digital camera (1,300 × 1,030 pixels resolution and 8-bit quantization) after addition of 1 μL of the propidium iodide aqueous solution (200 μmol L^–1) in 5 μL of total solution volume. Images were obtained using a 40X objective lens and a green filter effect in red. The spectral range was set with the excitation at 538 nm within the visible spectrum in order to produce orange-red fluorescence centered at 619 nm, which was processed using the Axio 4.7 software.

Nine-thousand P. gallinaceum mature sporozoites were recovered from the salivary glands of Ae. aegypti and incubated in 50 μL of the PBS solution, with 40 μmol L⁻¹ digitonin (positive control), 60 μmol L⁻¹ peptides or negative controls, at 37 °C for 1 h. Cell membrane integrity was then monitored using a Carl Zeiss inverted fluorescence microscope (model Observer Axio Vision A.1) coupled to an image capture Zeiss AxioCam HR digital camera (1300 × 1030 pixels resolution and 8-bit quantization) after addition of 1 μL of the propidium iodide aqueous solution (200 μmol L⁻¹) in 5 μL of total solution volume. Images were obtained using a 40× objective lens and a green filter effect in red. The spectral range was set with the excitation at 538 nm within the visible spectrum in order to produce orange-red fluorescence centered at 619 nm, which was processed using the Axio 4.7 software.