For immunoblot, protein samples were resolved by SDS-PAGE, transferred to PVDF membranes (Millipore), and incubated with primary antibodies: anti-Ccnd1 (monoclonal DCS-6, BD biosciences, 1:500), anti-Gabra4 (polyclonal, AB5459, Merck, 1:1000, and polyclonal 12979-1-AP, Proteintech, 1:500), anti-Flag (polyclonal, 11508721, Invitrogen, 1:1000), anti-HA (rat monoclonal 3F10, Roche #11867431001, 1:5000), anti-TFR (monoclonal H68.4, Invitrogen #13-6800, 1:1,000) and anti-GST (goat polyclonal, Amersham #27-4577, 1:2000). Appropriate peroxidase-linked secondary antibodies (GE Healthcare UK Ltd) were detected using the chemiluminescent HRP substrate Immobilon Western (Millipore). Chemiluminescence was recorded with a ChemiDoc-MP imaging system (BioRad).
Anti ccnd1
Manufactured by BD
Anti-Ccnd1 is a laboratory reagent used for the detection and analysis of Cyclin D1 (Ccnd1) protein expression in biological samples. It is a specific antibody that binds to Ccnd1, a key regulator of the cell cycle. Anti-Ccnd1 can be used in various analytical techniques such as Western blotting, immunohistochemistry, and flow cytometry to quantify and localize Ccnd1 in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Pvdf membrane, by Merck Group (2 mentions)
Immobilon western, by Merck Group (2 mentions)
Anti flag, by Thermo Fisher Scientific (2 mentions)
Dcs 6, by BD (2 mentions)
Chemidoc mp imaging system, by Bio-Rad (2 mentions)
Anti gabra4, by Merck Group (2 mentions)
Appropriate peroxidase linked secondary antibodies, by Merck Group (2 mentions)
2 protocols using anti ccnd1
1
Immunoblotting Protein Detection Protocol
2
Immunoblotting Protein Detection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For immunoblot, protein samples were resolved by SDS-PAGE, transferred to PVDF membranes (Millipore), and incubated with primary antibodies: anti-Ccnd1 (monoclonal DCS-6, BD biosciences, 1:500), anti-Gabra4 (polyclonal, AB5459, Merck, 1:1000, and polyclonal 12979-1-AP, Proteintech, 1:500), anti-Flag (polyclonal, 11508721, Invitrogen, 1:1000), anti-HA (rat monoclonal 3F10, Roche #11867431001, 1:5000), anti-TFR (monoclonal H68.4, Invitrogen #13-6800, 1:1,000) and anti-GST (goat polyclonal, Amersham #27-4577, 1:2000). Appropriate peroxidase-linked secondary antibodies (GE Healthcare UK Ltd) were detected using the chemiluminescent HRP substrate Immobilon Western (Millipore). Chemiluminescence was recorded with a ChemiDoc-MP imaging system (BioRad).
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