Cell dyn 3700 system

For measurement of hematological parameters, blood-EDTA samples were collected from the retrobulbar venous plexus of 26 Ndst4^−/− and 27 WT mice at 8 weeks of age for determination of complete blood counts, including leukocyte differentiation. Hematological parameters were measured by an automatic electronic cell counter, Abbott CELL-DYN 3700 System (Santa Clara, CA, USA) (Supplementary Table S3).

Macrophages were depleted by a single tail vein administering 150 μL clodronate liposomes (Clodronate Liposomes. org, Netherlands). Then mice were challenged with LAC (3 × 10⁷ CFU) by intraperitoneal injection. Infected-mice were sacrificed at 0, 24, 48, 96, and 120 h later. Macrophage depletion was confirmed by flow cytometry in peritoneal lavage fluid.
Mice were rendered neutropenic by a single tail vein injection cyclophosphamide (CTX, 200 mg/kg). Peritoneal lavage fluid was collected and the percentage of macrophage was detected by flow cytometry after the mice were intraperitoneal administered with LAC (3 × 10⁷ CFU). Blood was collected from the retro-orbital sinuses of anesthetized to determine the extent of neutropenia at 0, 24, 48, 96, and 120 h after injection. The blood was analyzed with an Abbott Cell-Dyn 3700 system.

Blood cell counts and serum biochemical tests were performed when patients were diagnosed. Peripheral venous blood was collected using EDTA-anticoagulated Vacutainer CPT tubes (BD Biosciences, San Diego, CA, USA). Blood sera were collected using serum separator tube (BD Biosciences). At the time of blood sampling, no subjects had an acute infection or were taking any medication known to influence blood cell components or serum biochemistry profiles. Blood and serum samples were sent to the laboratory and processed within 1 hour. Blood cell counts were required on a hematology analyzer (Abbott CELL DYN 3700 System, Ramsey, Minnesota 55303, USA). Serum total protein (TP), ALB, globulin (GLB), TBIL, DBIL, and IBIL were analyzed on an Architect C 16000 (Abbott) device at the biochemistry laboratory of Sichuan Provincial People's Hospital. The NAR, NTBR, and NIBR were calculated as the ratio of neutrophil counts (×10⁹/L)-to-ALB (g/L), neutrophil counts (×10⁹/L)-to-TBIL (μmol/L), and neutrophil counts (×10⁹/L)-to-IBIL(μmol/L), respectively.

We used comprehensive metabolic panels to test serum samples collected in 2.5-mL Z Serum Separator Clot Activator VACUETTE Tubes (Greiner Bio-One, Monroe, NC, USA) by using a Piccolo Xpress Chemistry Analyzer and Piccolo General Chemistry 13 Panel (Abbott Point of Care, Princeton, NJ, USA). Complete blood counts were performed on whole blood collected in 1.2-mL S-Monovette K3 EDTA Tubes (Sarstedt, Nümbrecht, Germany) by using a CELL-DYN 3700 system (Abbott Point of Care).

The peripheral counts of blood cells were measured by certified technologists using CELL-DYN 3700 system (Abbott Laboratories, Illinois, USA) in the DFTJ cohort and Beckman Coulter Counter (Beckman Coulter Inc., Brea, CA, USA) in the NHANES 1999–2014. The SII level was calculated for each participant as follows: SII (× 10⁹/L) = neutrophil count (× 10⁹/L)/lymphocyte count (× 10⁹/L) × platelet count (× 10⁹/L)^{17 (link)}.

Seven Yorkshire pigs (Midwest Research Swine, Gibbon, MN) weighing 68.0 ± 4.9 kg were used in this study. To ensure health of the animal and the kidney, blood samples were taken prior to euthanasia for analysis of blood chemistry and complete blood count (CBC). All procedures were approved by the Regulatory Research Compliance Division of the US Army Institute of Surgical Research (USAISR). Specifically, a tissue sharing request to the Animal Care and Use Committee from USAISR was completed. This research was conducted in compliance with the Animal Welfare Act, the implementing Animal Welfare Regulations, and the principles of the Guide for the Care and Use of Laboratory Animals, National Research Council. The facility's Institutional Animal Care and Use Committee approved all research conducted in this study. The facility where this research was conducted is fully accredited by AAALAC International. For protein, creatinine, and BUN measurement, blood was collected into a BD Vacutainer tube with Lithium Heparin (Becton Dickinson and Company, Franklin Lakes, NJ) and centrifuged at 1,200 g for 10 min. Samples were analyzed on a Siemens Dimension® Xpand™ Plus clinical Chemistry system. For CBC analysis, specimens were collected in a BD Vacutainer tube containing K2 EDTA (Becton Dickinson and Company, Franklin Lakes, NJ) and analyzed with the Abbott Cell-Dyn® 3700 system.