Log In Sign up
The largest database of trusted experimental protocols

Fe sod

Manufactured by Agrisera
Visit Supplier
Sourced in Sweden

Fe-SOD is a lab equipment product that functions as a superoxide dismutase (SOD) enzyme containing iron (Fe) as the metal cofactor. It catalyzes the dismutation of superoxide radicals into oxygen and hydrogen peroxide, playing a crucial role in the defense against oxidative stress.

Automatically generated - may contain errors

Lab products found in correlation

Mn sod, by Agrisera (1 mentions) Anti rabbit igg horseradish peroxidase conjugate, by Merck Group (1 mentions) 3 3 diaminobenzidine (dab), by Merck Group (1 mentions) Catalase, by Merck Group (1 mentions) Menadione, by Merck Group (1 mentions) Tiron, by Merck Group (1 mentions)

2 protocols using fe sod

1

Antioxidant Enzyme Profiling in Plants

Check if the same lab product or an alternative is used in the 5 most similar protocols
The procedure of protein extraction, electrophoresis, electrotransfer, and Western blot were the same as described in Section 4.3.4. The antibodies directed against particular enzymes were commercially available and produced by Agrisera® company (Vännäs, Sweden): APX (AS08368), CAT (AS09501), Fe-SOD (AS06125), Mn-SOD (AS09524), Cu/Zn-SOD (AS10652), GR (AS06181), and GPX (AS04055). The primary antibody was diluted 1:4000, and the antigen–antibody complexes were detected using a secondary anti-rabbit IgG–horseradish peroxidase conjugate diluted 1:20000 (Sigma, St. Louis, MO, USA, currently member of Merck Group, Dormstadt, Germany). The measurements were carried out in three replicates at each time-point.
Augustyniak A., Pawłowicz I., Lechowicz K., Izbiańska-Jankowska K., Arasimowicz-Jelonek M., Rapacz M., Perlikowski D, & Kosmala A. (2020). Freezing Tolerance of Lolium multiflorum/Festuca arundinacea Introgression Forms is Associated with the High Activity of Antioxidant System and Adjustment of Photosynthetic Activity under Cold Acclimation. International Journal of Molecular Sciences, 21(16), 5899.
+ Open protocol
+ Expand
2

Antioxidant Enzyme Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Menadione, NBT, DAB, catalase and Tiron (4, 5-Dihydroxy-1, 3-benzenedisulfonic acid disodium salt) and Premix-BCIP/NBT solution were obtained from Sigma–Aldrich (St. Louis, MO, USA). Primary antibodies for GO, CAT, HPR, GR, and SOD (CuZn-SOD and Fe-SOD) and secondary antibody goat anti-rabbit IgG conjugated with alkaline phosphatase were from Agrisera AB (Vännäs, Sweden). All the chemicals were of analytical grade.
Bapatla R.B., Saini D., Aswani V., Rajsheel P., Sunil B., Timm S, & Raghavendra A.S. (2021). Modulation of Photorespiratory Enzymes by Oxidative and Photo-Oxidative Stress Induced by Menadione in Leaves of Pea (Pisum sativum). Plants, 10(5), 987.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!