Fastking first strand cdna synthesis mix

Total RNAs were extracted from lung tissues using TRIZOL reagent (Invitrogen, Carlsbad, CA, USA). By using the template of cDNAs that reversely transcribed by FastKing First-strand cDNA Synthesis Mix (Tiangen, Beijing, China), qRT-PCR was performed on MX3000P Real-Time PCR instrument (Agilent, Santa Clara, CA, USA). The qRT-PCR program was an initial 95°C for 3 min and 40 cycles of 95°C for 15 s and 62°C for 40 s. The relative expression of RKIP was calculated by the 2^-∆∆Ct method. GAPDH was used as the internal control. The primers used in qRT-PCR are listed in Table 1.

Total RNA was extracted from cells using TRIzol reagent (Invitrogen, CA, USA) and was reverse-transcribed using FastKing First-strand cDNA Synthesis Mix (Tiangen, China). RT-qPCR was performed using SYBR Green qPCR Kit (Lifeint, Xiamen, China) on Mx3000P system (Stratagene, Carlsbad, CA, USA). The RT-qPCR program was an initiative of 95°C for 3 min, followed by 40 cycles of 95°C for 15 s and 62°C for 40 s. GAPDH was used as the internal control, and relative mRNA expression of CPSF7 was calculated by the 2^−∆∆Ct method. The primers used in RT-qPCR included CPSF7-F, 5′–GCT GAC GAG GAG TTC AAC CA–3′, CPSF7-R, 5′–ACG GCA GCT CGT CTA TTA CG–3′; GAPDH-F, 5′–ACT CAC GGC AAA TTC AAC GG–3′, and GAPDH-R, 5′–AGT TGG GAT AGG GCC TCT CTT G–3′.