Evom voltohmmeter system

Astrocyte cells (C6, rat astrocyte cell line) and endothelial cells (bEnd.3, mouse endothelial cell line) were from ATCC (Manassas, VA, USA). Cell culture inserts for 24-well plates (transparent PET membrane transwells, 0.4 µm pore size) were obtained from Dominique Dutscher (Alsace, France). Imaging Plate FC, 24-well plates, TC-surface was purchased from Ozyme (Saint Quentin Yvelines, France). The EVOM voltohmmeter system was from World Precision Instruments (Hertfordshire, UK). ZO-1 antibodies were from Life Technologies (cat 40-2200; Saint Aubin, France) and claudin-5 antibodies were from ABCAM (ab15106; Paris, France). P-gp and MRP-1 antibodies were from GeneTex (GTX23364; San Antonio, TX, USA) and Santa Cruz Biotechnology (sc-13960; Dallas, TX, USA), respectively. Standard protein and all compounds for Ringer HEPES buffer, BCECF-AM, probenicid, verapamil, sodium fluorescein (Na-Fl), were from Sigma-Aldrich (St. Quentin Fallavier, France). Rhodamine-123 was from Thermo Fisher Scientific (Eugene, OR, USA). Hydralazine, MTT kit and Dulbecco’s Modified Eagle’s Medium (DMEM) were also from Sigma-Aldrich. Antibodies and reagents for the detection of HIF-1α were products of R&D systems (Lille, France). YC-1 was purchased from VWR International (Fontenay-sous-Bois, France).

Hydralazine, BCECF-AM, probenecid, verapamil and Na-F were purchased from Sigma-Aldrich (St Quentin Fallavier, France). MTT (methyl-thiazolyl-tetrazolium) kit and DMEM (Dulbecco’s Modified Eagle’s Medium) were also purchased from Sigma. All reagents for the LDH release test were purchased from Promega Corporation (Madison, USA). Antibodies and reagents for the detection of HIF-1α were products of R&D systems (Lille, France). All compounds for Ringer-Hepes buffer were purchased from Sigma.
Occludin and ZO-1 antibodies were purchased from Life Technologies (Saint Aubin, France), Pgp and MRP-1 antibodies were purchased from GeneTex (San Antonio, Texas, U.S.A) and Santa Cruz Biotechnology (Dallas, Texas, U.S.A), respectively.
bEnd.3 cells and C6 cells were obtained from the ATCC (Manassas, VA, USA).
Cell culture inserts for 24-well (0.4 μm pore diameter size, transparent PET membrane) were purchased from Corning distributors (Sigma). EVOM voltohmmeter system was purchased from World Precision Instruments (Hertfondsire, UK).

Astrocyte cells (human astrocytes) were from ScienCell Research Laboratory (Carlsbad, CA, USA) and endothelial cells (HBEC-5i, human brain endothelial cells) were obtained from ATCC (Manassas, VA, USA). Specific inserts for 24-well (transparent PET membrane, 0.45-µm pore diameter size) were from D. Dutscher (Alsace, France). EVOM voltohmmeter system was purchased from World Precision Instruments (Hertfondsire, UK). Occludin antibody was from Life Technologies (Saint Aubin, France), and ZO-1 was from GeneTex (San Antonio, TX, USA). Pgp, claudin-5, BCRP and MRP-1 antibodies came from Santa Cruz Biotechnology (Dallas, TX, USA). All compounds for Ringer HEPES buffer were from Sigma-Aldrich (St Quentin Fallavier, France) as well as BCECF-AM, verapamil, KO143, probenecid, Na-Fl, DMEM-F12 (Dulbecco’s Modified Eagle’s Medium) and methyl-thiazolyl-tetrazolium (MTT). Rhodamine-123 was from Thermo Fisher Scientific (Waltham, MA, USA).