Silencer select

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany, Japan, France, China

Silencer Select is a lab equipment product designed for gene silencing. It provides a solution for selectively reducing the expression of target genes in cellular systems.

Automatically generated - may contain errors

Lab products found in correlation

341 protocols using silencer select

siRNA Transfection Assay for Centrosomal Proteins

Cited 4 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Transfections of siRNA were performed using Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions. 30,000–40,000 cells were seeded per well in a 24-well plate and analyzed 72 h after the initial transfection. The following siRNAs (50 nM final concentration) were used: siLuciferase (siControl), 5′-UCGAAGUAUUCCGCGUACG-3′ (Dharmacon ON-TARGET plus/Ambion Silencer Select; Knodler et al., 2010 (link)); siNek2, 5′-GAUGCAAUUUGGUCAUUAAUU-3′ and 5′-GAAAGGCAAUACUUAGAUGUU-3′ (Dharmacon ON-TARGET plus; Kim et al., 2015 (link)); siCep164, 5′-GGUGACAUUUACUAUUUCA-3′ (Ambion Silencer Select; Graser et al., 2007 (link)); siHsODF2-1, 5′-AGACUAAUGGAGCAACAAG-3′ (Ambion Silencer Select; Soung et al., 2009 (link)); siODF2-2, 5′-GGAUCUUUAUGUCGCUGAATT-3′ (Ambion Silencer Select); siKif24, 5′-GGAACACCCTGGAGAATAGTT-3′ and 5′-GAGTTGAGCTCTCCTTTGGTT (Dharmacon ON-TARGET plus; Kobayashi et al., 2011 (link)); and siC-Nap1 (human CEP250), 5′-GAGCAGAGCUACAGCGAAU-3′ and 5′-AAGCUGACGUGGUGAAUAA-3′ (Dharmacon ON-TARGET plus; Panic et al., 2015 (link)).

Viol L., Hata S., Pastor-Peidro A., Neuner A., Murke F., Wuchter P., Ho A.D., Giebel B, & Pereira G. (2020). Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis. The Journal of Cell Biology, 219(3), e201907136.

+ Open protocol

+ Expand

Knockdown of MLKL and RIPK3 in AML12 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

MLKL was knocked down by RNA interference using MLKL siRNA (Cat# 4390771, ID: s92950 Silencer Select, Ambion, Life Technologies Corporation, New York, NY, USA). Similarly, RIPK3 was knocked down by RNA interference using RIPK3 siRNA (Cat# 4390771, ID: s80754, Silencer Select, Ambion, Life Technologies Corporation, New York, NY, USA). Silencer Negative control (Cat# 4390843, Silencer Select, Ambion, Life Technologies Corporation, New York, NY, USA) was used as negative control and GAPDH (Cat# 4390849, Silencer Select, Ambion, Life Technologies Corporation, New York, NY, USA) was used as positive control. Briefly, AML12 cells were seeded in a 12 well plate, 24 h prior to the transfection treatment. By following the manufacturer’s guidelines, siRNA was mixed with Lipofectamine RNAiMAX (Cat# 13778-150, Invitrogen, Life Technologies Corporation, NY, USA) in Opti-MEM I (Cat# 31985-070, Gibco, Life Technologies Corporation, NY, USA) and incubated for 15 min prior to transfer of the cells to 37 °C for 48 h. Then the transfected cells were prepared for FFA treatment followed by OGD exposure.

Baidya R., Gautheron J., Crawford D.H., Wang H, & Bridle K.R. (2021). Inhibition of MLKL Attenuates Necroptotic Cell Death in a Murine Cell Model of Hepatic Ischaemia Injury. Journal of Clinical Medicine, 10(2), 212.

+ Open protocol

+ Expand

RICTOR Silencing for Melanoma Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Due to the lack of specific inhibitors, RNA interference was used to suppress RICTOR expression. MelIM, MelJU and B16 were transiently transfected with two different siRNA sequences targeting human [(Silencer® Select; s48410 (=RICTOR si1), s226000 (=RICTOR si2)); Invitrogen, Waltham, MA, USA] or murine [(Silencer® Select; s95670 (RICTOR si1), s95672 (RICTOR si2)); Invitrogen, Waltham, MA, USA] RICTOR and scrambled siRNA control [(Silencer® Select; scrambled siRNA = ctrl. Si); Invitrogen, Waltham, MA, USA] using Lipofectamine RNAiMAX transfection reagent (Invitrogen, Waltham, MA, USA). Briefly, pre-plated cells were incubated with transfection mixture [Opti-MEM® (Gibco, Waltham, MA, USA), Lipofectamine 2.5% vol/vol, siRNA 50 nM] for 6 hours. 48 hours after transfection knock-down efficiency was confirmed by Western blotting and tumor cells were processed for further experiments.

Schmidt K.M., Dietrich P., Hackl C., Guenzle J., Bronsert P., Wagner C., Fichtner-Feigl S., Schlitt H.J., Geissler E.K., Hellerbrand C, & Lang S.A. (2018). Inhibition of mTORC2/RICTOR Impairs Melanoma Hepatic Metastasis. Neoplasia (New York, N.Y.), 20(12), 1198-1208.

+ Open protocol

+ Expand

Targeted Protein Silencing Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

STIL (Thermo Fisher Scientific, Silencer Select #s12863),
HsSAS6 (Thermo Fisher Scientific, Silencer Select #s46487),
Negative control (Thermo Fisher Scientific, Silencer Select #4390843).

Takao D., Watanabe K., Kuroki K, & Kitagawa D. (2019). Feedback loops in the Plk4–STIL–HsSAS6 network coordinate site selection for procentriole formation. Biology Open, 8(9), bio047175.

+ Open protocol

+ Expand

Transient SNAI1 Modulation in TDECs and MSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

According to the manufacturer’s instruction, TDECs or MSCs were transiently transfected with 50 nM of SNAI1 siRNA (Silencer Select, Life Technologies) or pCMV-Snai1 (Silencer Select, Life Technologies) for 48 h using Lipofectamine 2000 Transfection Reagent (Life Technologies, Shanghai, China). The cells were also treated with a scrambled siRNA targetting Firefly luciferase or pCMV (Silencer Select, Life Technologies) as a negative control. The efficiency of Snai1 silencing or overexpression was determined by western blot.

Zhang Y.K., Wang H., Guo Y.W, & Yue Y. (2019). Novel role of Snail 1 in promoting tumor neoangiogenesis. Bioscience Reports, 39(5), BSR20182161.

+ Open protocol

+ Expand

Silencing PANCR and PITX2c in cardiomyocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

100 pmol of a custom PANCR siRNA (Ambion silencer select, Supplemental Table 1), 33 pmol of each of 3 PITX2c siRNAs (Ambion silencer select cat#4392420, ids: S10557, S10558, and S10559), or 100 pmol of a control scramble siRNA (Ambion silencer select cat # 4390843) were transfected in differentiated H9 induced cardiomyocytes using the RNAiMax (Invitrogen) transfection reagent according to manufactures specifications. The siRNA complexes were incubated with cells for 48 hrs followed by RNA isolation, cDNA preparation, and qRT-PCR, as described above.

Gore-Panter S.R., Hsu J., Barnard J., Moravec C.S., Van Wagoner D.R., Chung M.K, & Smith J.D. (2016). PANCR, the PITX2 Adjacent Noncoding RNA, Is Expressed in Human Left Atria and Regulates PITX2c Expression. Circulation. Arrhythmia and electrophysiology, 9(1), 10.1161/CIRCEP.115.003197 e003197.

+ Open protocol

+ Expand

Transient gene knockdown and overexpression

Check if the same lab product or an alternative is used in the 5 most similar protocols

For transient gene knockdown, cells were reverse transfected with siRNAs in 24-well plates, using Lipofectamine RNAiMax (Invitrogen) according to the manufacturer’s instructions, except that McCoy’s 5A was used as the dilution medium. The medium was changed the following day, and the cells were assayed 48 h posttransfection. siRNA duplexes were used at the following concentrations: siRNA Universal Negative Control #1 (Sigma-Aldrich) and Mic10 (5′-CGGAUGCGGUCGUGAAGAUTT-3′; Eurofins Genomics) at 100 nM; Mic13 (Ambion, Silencer Select catalog no. s195661), Mic26 (Ambion, Silencer Select catalog no. s35601), and Mic27 (Ambion, Silencer Select catalog no. s225655) at 20 nM. For transient overexpression, cells were seeded in 24-well plates 1 day before transfection with 0.5 μg of plasmid DNA, using jetPRIME (Polyplus Transfection) according to the manufacturer’s instructions. Cells were assayed 24 h posttransfection. The expression plasmids pcDNA3.1(+)-MINOS1-DYK (GenScript, ORF cDNA clone OHu15514) and pCMV6-ATP5MD-Myc-DDK (OriGene, TrueORF Gold clone RC203316) were used to express FLAG-tagged Mic10 and DAPIT, respectively. Negative-control cells were transfected with pcDNA3.1(+) (Invitrogen). For immunofluorescence, cells were seeded in wells containing glass coverslips.

Carvalho F., Spier A., Chaze T., Matondo M., Cossart P, & Stavru F. (2020). Listeria monocytogenes Exploits Mitochondrial Contact Site and Cristae Organizing System Complex Subunit Mic10 To Promote Mitochondrial Fragmentation and Cellular Infection. mBio, 11(1), e03171-19.

+ Open protocol

+ Expand

HaCaT Knockdown via BLMH siRNAs

Check if the same lab product or an alternative is used in the 5 most similar protocols

To generate a BLMH knockdown in HaCaT cells, small interfering RNAs (siRNAs) from Silencer Select (Invitrogen) was purchased (BLMH specific siRNAs assay ID s2001 and s2002, 4392420, and Silencer Select Negative Control 4390843). siRNAs (10 nM) were mixed with 1 ul Lipofectamine RNAiMAX (Invitrogen) and 98 ul Opti-MEM Reduced Serum Medium (Gibco) and incubated for 30 minutes at room temperature. The siRNA-lipofectamine mix was added dropwise to HaCaT cells plated in 12 well culture plates and incubated overnight. After transfection, the cells were washed with fresh culture media and rested for 4 hours pending further stimulation. A viability check was performed 24 hours after the siRNA transfection using Trypan blue staining in a Cedex HiRes (Ninolab) cell counter.

Riise R., Odqvist L., Mattsson J., Monkley S., Abdillahi S.M., Tyrchan C., Muthas D, & Yrlid L.F. (2019). Bleomycin hydrolase regulates the release of chemokines important for inflammation and wound healing by keratinocytes. Scientific Reports, 9, 20407.

+ Open protocol

+ Expand

Knockdown of CD26 and CASP1 in Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

For CD26 KD analysis, H2452 cells were grown in 12‐well tissue culture‐treated plates (Corning, New York, NY) and transfected with either 20 pmol/L of small interfering RNA (siRNA) targeted CD26 (Silencer^® Select, s4254 as #1 and s4255 as #2, Ambion, Austin, TX), or control siRNA (Silencer^® Select, Negative Control #1) using Lipofectamine RNAiMAX (Invitrogen). For CASP1 KD analysis, THP‐1 cells were grown in 12‐well tissue culture‐treated plates (Corning) and transfected with either 100 pmol/L of small interfering RNA (siRNA) targeted CASP1 (Silencer^® Select, s2407) using Viromer^® GREEN (Lipocalyx, Weinbergweg, Germany). After 48 h, cells were harvested and used for the individual experiments.

Horio D., Minami T., Kitai H., Ishigaki H., Higashiguchi Y., Kondo N., Hirota S., Kitajima K., Nakajima Y., Koda Y., Fujimoto E., Negi Y., Niki M., Kanemura S., Shibata E., Mikami K., Takahashi R., Yokoi T., Kuribayashi K, & Kijima T. (2020). Tumor‐associated macrophage‐derived inflammatory cytokine enhances malignant potential of malignant pleural mesothelioma. Cancer Science, 111(8), 2895-2906.

+ Open protocol

+ Expand

Engineered Viral Vectors for FHF2 Overexpression and Silencing

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Overexpression vectors for FHF2A and FHF2B were created from the G0692 bicistronic internal ribosome entry sequence (IRES) AAV shuttle vector from University of Iowa Viral Vector Core. The genes for these proteins were subcloned into the first cistron. The Cheriff-GFP protein was subcloned into the second cistron. CheRiff-eGFP was taken from the DRH313FCK-CheRiff-eGFP plasmid originally created in the Cohen lab and sourced from Addgene (Watertown, MA, plasmid #51693) (41 (link)). The translated proteins were full FHF2A, FHF2B and CheRiff-eGFP fusion protein.
miRNA vectors were created with the same shuttle vector backbone. miRNAs were subcloned into the first cistron. The miRNAs targeting FHF2A and all isoforms of FHF2 (FHF2core) were derived from commercially available siRNA sequences (Silencer Select, ThermoFisher, Waltham MA). The scrambled sequence miRNA was from the G0784 vector from University of Iowa viral vector core. The miRNA-FHF2A and scrambled sequences were the same as used previously (25 (link)). The miRNA-FHF2core for this study was created from a different siRNA that targets rat FGF13 (Silencer Select, s136669, ThermoFisher) in the same manner as described previously (25 (link); 42 (link)).

Effraim P.R., Estacion M., Zhao P., Sosniak D., Waxman S.G, & Dib-Hajj S.D. (2022). Fibroblast growth factor homologous factor 2 attenuates excitability of DRG neurons. Journal of Neurophysiology, 128(5), 1258-1266.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!