C57bl 6j

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C57BL/6J is a mouse strain commonly used in biomedical research. It is a common inbred mouse strain that has been extensively characterized.

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Wild-type C57BL/6J mice (525 citations) C57BL/6J-Sting1gt/J (11 citations) Heterozygous K18-hACE2 C57BL/6J mice (7 citations) WT C57BL/6J female mice (7 citations) C57BL/6J-Tmem173gt/J mice (4 citations) C57BL/6J mouse pups (3 citations) C57BL/6J nursing mothers (2 citations) C57BL/6J embryos (2 citations) C57BL/6J-Smsem2Lutzy/J (2 citations) C57BL/6J ob/ob (2 citations)

4 187 protocols using c57bl 6j

Characterizing Obesity-Induced Gene Changes

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Mice used in this study were purchased from the Jackson Laboratory (JAX, Bar Harbor, ME, United States). In detail, 18-week-old male high-fat diet-induced obese (DIO) mice (12 weeks of HFD) (Stock Number: 380,050, Strain Name: C57BL/6J DIO, the Jackson Laboratory) and 18-week-old male ND mice (Stock Number: 000,664, Strain Name: C57BL/6J, the Jackson Laboratory) were used for microarray assay and comparison of gene characteristics. Nine-week-old male DIO mice (3 weeks of HFD) were generated via 3 weeks of HFD in 6-week-old wild-type (WT) mice (Stock Number: 000,664, Strain Name: C57BL/6J, the Jackson Laboratory), and 9-week-old male ND mice were generated via 3 weeks normal diet (ND) in 6-week-old WT mice (Stock Number: 000,664, Strain Name: C57BL/6J, the Jackson Laboratory). If not otherwise specified, mouse bone marrow mesenchymal stem cells (BMSCs) were obtained from 4- to 6-week-old male WT mice (Stock Number: 000,664, Strain Name: C57BL/6J, the Jackson Laboratory). All in vivo experiments in this study followed the Tufts University Institutional Animal Care and Use Committee guidelines.

Hu Z., Qiu W., Yu Y., Wu X., Fang F., Zhu X., Xu X., Tu Q., Van Dyke T.E., Morgan E.F, & Chen J. (2022). Identification and Characterization of a Novel Long Noncoding RNA that Regulates Osteogenesis in Diet-Induced Obesity Mice. Frontiers in Cell and Developmental Biology, 10, 832460.

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Genetically Modified Mouse Strains

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Male and female C57BL/6J (Jax stock #000664), C57BL/6J Nos2^-/- (B6.129P2-Nos2^tm1Lau/J; Jax stock #002609), C57BL/6J Cybb^-/- (Jax stock #002365; B6.129S-Cybb^tm1Din/J), C57BL/6J Ccr2^-/- (Jax stock #004999; B6.129S4-Ccr2^tm1Ifc/J), C57BL/6J Il1r1^-/- (Jax stock #003245; B6.129S7-Il1r1^tm1Imx/J) and C57BL/6J Tlr2^-/- (Jax stock #004650; B6.129-Tlr2^tm1Kir/J) mice were purchased from Jackson Laboratory (Bar Harbor, Maine) and each colony was bred and maintained in a conventional animal facility. Pups were housed with the dam until 3-weeks of age. The Institutional Animal Care and Use Committee of New York University Medical Center approved all animal experiments.

Lokken-Toyli K.L., Aggarwal S.D., Bee G.C., de Steenhuijsen Piters W.A., Wu C., Chen K.Z., Loomis C., Bogaert D, & Weiser J.N. (2024). Impaired upper respiratory tract barrier function during postnatal development predisposes to invasive pneumococcal disease. PLOS Pathogens, 20(5), e1012111.

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Acute Dorsal Hippocampal Slice Preparation

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Acute 300 µm thick coronal dorsal hippocampal slices were cut from C57Bl6/J (Jackson Laboratories, Bar Harbor, ME) (n = 70), Tg(Chrna2-Cre)OE25Gsat/Mmucd (RRID:MMRRC_036502-UCD, on C57Bl6/J background) (n = 18), sst ^{tm3.1 (flop) Zjh}/J (RRID:Cat_JAX:028579, RRID:IMSR_JAX:028579 on C57Bl6/J background) (n = 2), and Tg(Vipr2-cre)KE2Gsat/Mmucd (RRIP: MMRRC_034281-UCD) × Dlx5/6-Flpe (Tg(mI56i-flpe)39Fsh/J), (RRID:IMSR_JAX:010815) on C57Bl6/J background (n = 1) mice of both sexes (postnatal day 52–86). Animals were anaesthetized with a ketamine, xylasine, pypolphene cocktail (0.625, 6.25, 1.25 mg/ml, respectively, 10 µl/g body weight) and then decapitated or perfused with ice-cold cutting solution containing (in mM): sucrose, 205.2; KCl, 2.5; NaHCO₃, 26; CaCl₂, 0.5; MgCl₂, 5; NaH₂PO₄, 1.25; and glucose, 10, bubbled with 95% O₂ and 5% CO₂. The brain was quickly removed into ice-cold cutting solution, and coronal slices containing the dorsal hippocampus were cut using a Leica vibratome (VT1200S, Leica, Wetzlar, Germany) and placed in a submerged-type chamber in ACSF containing (in mM): NaCl, 126; KCl, 2.5; NaHCO₃, 26; CaCl₂, 2; MgCl₂, 2; NaH₂PO₄, 1.25; glucose, 10 saturated with 95% O₂ and 5% CO₂ (pH = 7.2–7.4) at 36°C, which was then gradually cooled down to 22–24°C. Recordings were carried out in the same ACSF 32–33°C, slices were kept up to 6 hr.

Karlocai M.R., Heredi J., Benedek T., Holderith N., Lorincz A, & Nusser Z. (2021). Variability in the Munc13-1 content of excitatory release sites. eLife, 10, e67468.

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Acute Hippocampal Slice Preparation

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Acute 300 µm thick coronal dorsal hippocampal slices were cut from C57Bl6/J (Jackson Laboratories, Bar Harbor, ME, USA) (n = 70),Tg(Chrna2-Cre)OE25Gsat/Mmucd, (RRID:MMRRC_036502-UCD, on C57Bl6/J background) (n = 18), sst tm3.1 (flop) Zjh /J, (RRID: Cat# JAX:028579, RRID:IMSR_JAX:028579 on C57Bl6/J background) (n = 2) and Tg(Vipr2cre)KE2Gsat/Mmucd, (RRIP: MMRRC_034281-UCD) x Dlx5/6-Flpe (Tg(mI56i-flpe)39Fsh/J, (RRID:IMSR_JAX:010815) on C57Bl6/J background (n = 1) mice of both sexes (postnatal day 52 -86).
Animals were anaesthetized with a ketamine, xylasine, pypolphene cocktail (0.625, 6.25, 1.25 mg / ml respectively, 10 µl / g body weight) then decapitated, or perfused with ice cold cutting solution containing (in mM): sucrose, 205.2; KCl, 2.5; NaHCO3, 26; CaCl2, 0.5; MgCl2, 5; NaH2PO4, 1.25; and glucose, 10, bubbled with 95% O2 and 5% CO2. The brain was quickly removed into ice cold cutting solution and coronal slices containing the dorsal hippocampus were cut using a Leica vibratome (VT1200S, Leica, Wetzlar, Germany) and placed in a submerged-type chamber in ACSF containing (in mM): NaCl, 126; KCl, 2.5; NaHCO3, 26; CaCl2, 2; MgCl2, 2; NaH2PO4, 1.25 ; glucose, 10 saturated with 95% O2 and 5% CO2 (pH = 7.2 -7.4) at 36°C, which was then gradually cooled down to 22 -24°C.
Recordings were carried out in the same ACSF 32 -33 o C, slices were kept up to 6 hours.

Karlócai M.R., Herédi J., Benedek T., Holderith N., Lőrincz A., & Nusser Z. (2021). Diversity of excitatory release sites.

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Transgenic Mouse Models for Immunological Research

Cited 1 time

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All animal work was performed in accordance with an animal protocol approved by the institutional Animal Care and Use Committee at WCMC (Protocol number 0806–762A). Female C57BL/6J (catalogue number 000664) mice were purchased from The Jackson Laboratory (Bar Harbor, Maine). OT-I mice^{25 (link)} on C57BL/6J (C57BL/6-Tg(TcraTcrb)1100Mjb/J, catalogue number 003831) and Thy1.1^+/+ mice on C57BL/6J (B6.PL-Thy1^a/CyJ, catalogue number 000406) were purchased from The Jackson Laboratory and bred in-house to produce OT-I⁺/Thy1.1^+/+ and OT-I⁺/Thy1.1^+/− mice. Tcf7^GFP mice^{45 (link)} on C57BL/6J (B6(Cg)-Tcf7^tm1Hhx/J, catalogue number 030909) were purchased from The Jackson Laboratory and bred in-house to OT-I⁺/Thy1.1^+/+ and OT-I⁺/Thy1.1^+/− to produce Tcf7GFP⁺/OT-I⁺/Thy1.1^+/+, Tcf7GFP⁺/OT-I⁺/Thy1.1^+/−, and Tcf7GFP⁺OT-I⁺/Thy1.1^−/− mice.

Markowitz G.J., Ban Y., Tavarez D.A., Yoffe L., Podaza E., He Y., Martin M.T., Crowley M.J., Sandoval T.A., Gao D., Martin M.L., Elemento O., Cubillos-Ruiz J.R., McGraw T.E., Altorki N.K, & Mittal V. (2023). Deficiency of metabolic regulator PKM2 activates the pentose phosphate pathway and generates TCF1+ progenitor CD8+ T cells to improve checkpoint blockade. Research Square.

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Conditional Knockout of Gde2 in SOD1 ALS Mice

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Mice were maintained and used in accordance with approved Johns Hopkins University IACUC protocols. Gde2^+/− and Gde2^lox/+ mice with mixed backgrounds (129/SV x C57BL/6J) were out-crossed to C57BL/6J (Jackson Laboratories 000664) for four generations. Gde2^lox/+;ROSA:Cre^ERmice were maintained on a C57BL/6J background and crossed with Gde2^−/− for conditional ablation of Gde2. B6SJL-Tg(SOD1*G93A)1Gur/J mice were obtained from Jackson Laboratory (Stock# 002726), and maintained as a hemizygous line on a C57BL/6J background. Animals for 6 month motor neuron counts were pooled from 6 to 7 month old animals. Genotyping primers, competitive PCR analysis, and 4-OHT administration are as previously described [8 (link)], and B6SJL-Tg(SOD1*G93A)1Gur/J mice were genotyped as specified by Jackson Laboratory.

Cave C., Park S., Rodriguez M., Nakamura M., Hoke A., Pletnikov M, & Sockanathan S. (2017). GDE2 is essential for neuronal survival in the postnatal mammalian spinal cord. Molecular Neurodegeneration, 12, 8.

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Nicotinic Receptor Knockout Mice Behavior

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Subjects were naïve adult (8–10 weeks old), β2, α4, and α7 global knockout mice and their wildtype littermates as well as C57BL6/J mice (Jackson Laboratory, Bar Harbor, ME). The β2 (backcrossed to a C57BL6/J background, original breeding pairs provided by Dr. Arthur Beaudet, Baylor College of Medicine), α4, and α7 (backcrossed to a C57BL6/J background, breeding pair obtained from Jackson Laboratories) heterozygous male and female mice were bred in our animal colonies to obtain β2, α4, and α7 KO and WT mice and backcrossed to a C57BL6/J background. All mice were group housed with ad-libitum access to water and food in a colony room maintained on a 12h light/dark cycle. All training and testing occurred between 9:00 am and 6:00 pm. Behavioral procedures used in this study were approved by the Temple University Institutional Animal Care and Use Committee.

Kutlu M.G., Holliday E, & Gould T.J. (2015). High-affinity α4β2 nicotinic receptors mediate the impairing effects of acute nicotine on contextual fear extinction. Neurobiology of learning and memory, 128, 17-22.

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Generating Transgenic Mouse Lines

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We obtained the heterozygous Fos-CreER^T2 mice used in this study by crossing wild-type C57BL6/J (Jackson Laboratory Stock # 000664) and Fos-CreER^T2 (+/−) mice (Jackson Laboratory Stock # 021882). We obtained heterozygous Arc-CreER^T2 mice by crossing wild-type C57BL6/J and Arc-CreER^T2 (+/−) mice (Jackson Laboratory Stock # 022357). We obtained heterozygous Fos-tTA/Fos-shGFP (+/−) mice by crossing wild-type C57BL6/J and Fos-tTA/Fos-shGFP (+/−) mice (Jackson Laboratory Stock # 018306). Fos-CreER^T2 (+/−) and Ai9 ROSA-LSL-tdTomato (+/+) mice (Jackson Laboratory Stock # 007909) were crossed to generate Fos-CreER^T2 (+/−) × ROSA-LSL-tdTomato (+/−) mice . Fos-CreER^T2 (+/−) and Fos-tTA/Fos-shGFP (+/−) mice were crossed to generate Fos-CreER^T2 (+/−) × Fos-tTA/Fos-shGFP (+/−) mice. Arc-CreER^T2 (+/−) and Fos-tTA/Fos-shGFP (+/−) mice were crossed to generate Arc-CreER^T2 (+/−) × Fos-tTA/Fos-shGFP (+/−) mice. GAD2-IRES-Cre (+/+) mice were obtained from the Jackson Laboratory (Stock # 010802). Mice were singly housed in home cages on a 12-h light/dark cycle with food and water continuously available. The light cycle was from 8 AM to 8 PM. Six- to eight-week-old mice of both sexes underwent stereotaxic brain surgery. All of the animal procedures were approved by the Institutional Animal Care and Use Committee of the University of California, Riverside.

Kim W.B, & Cho J.H. (2020). Encoding of contextual fear memory in hippocampal–amygdala circuit. Nature Communications, 11, 1382.

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Murine Pain Behavior Protocols

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These experiments were approved by the Veterans Affairs Palo Alto Health Care System Institutional Animal Care and Use Committee (Palo Alto, CA, USA) and followed the animal subjects guidelines of the International Association for the Study of Pain. The work also conforms to the Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines (https://www.nc3rs.org.uk/arrive-guidelines). Three strains of mice were used in these experiments; 1) muMT mice lacking mature B cells and immunoglobulin, on a C57BL/6J congenic background (#002288, Jackson Laboratory, Bar Harbor, ME), 2) Nrf2 knockout mice on a C57BL/6J congenic background (#017009, Jackson Laboratory), and 3) wildtype C57BL/6J controls (#000664, Jackson Laboratory). Three month old male mice were used in all experiments and were housed 4 per group under pathogen-free conditions with soft bedding and were given food and water ad libitum, with a 12:12 light:dark cycle. During the experimental period the animals were fed Teklad lab rodent diet 2018 (Harlan Laboratories, Indianapolis, IN), which contains 1.0% calcium, 0.7% phosphorus, and 1.5 IU/g vitamin D3, and were kept under standard conditions with a 12-h light-dark cycle. Data collection was conducted blind to group assignment.

Guo T.Z., Shi X., Li W., Wei T., Kingery W.S, & Clark J.D. (2021). Dimethyl Fumarate Reduces Oxidative Stress and Pronociceptive Immune Responses in a Murine Model of Complex Regional Pain Syndrome. Anesthesia and analgesia.

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Transgenic Mouse Breeding and Behavior

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Male and female C57BL/6J, B6.129S2-Emx1^tm1(cre)Krj/J (Emx1-Cre), and B6;129P2-Pvalb^tm1(cre)Arbr/J (PV-Cre) mice were housed 2–5 per cage under a 14/10 hr light/dark cycle with access to food (Labdiet 5015) and water ad libitum unless stated otherwise. Emx1-Cre (obtained from breeding homozygous B6.129S2-Emx1^tm1(cre)Krj/J (Jackson) x C57BL/6J in house), C57BL/6J (The Jackson Laboratory, Bar Harbor, ME, USA), and PV-Cre (obtained from breeding homozygous B6;129P2-Pvalb^tm1(cre)Arbr/J x C57BL/6J mice in house) mice were at least 6 weeks of age prior to intracranial injections and at least 7 weeks of age prior to behavioral training. All surgical and behavioral experiments were performed during the light portion of the cycle. The Animal Care and Use Committee of the University of California San Diego approved all experiments and experiments were conducted according to the NIH guidelines.

Baltz E.T., Yalcinbas E.A., Renteria R, & Gremel C.M. (2018). Orbital frontal cortex updates state-induced value change for decision-making. eLife, 7, e35988.

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