Ir affinity 1 spectrometer

Semipreparative HPLC (Agilent Technologies, 1260 Infinity II series) was performed using an ODS column (YMC-pack ODS-A, 10 mm × 250 mm, 5 μm). Column chromatography (CC) was performed over silica gel (200–300 mesh; Qingdao Marine Chemical Group Co., Qingdao, China) and Sephadex LH-20 (Amersham Biosciences Inc., Piscataway, NJ, USA) and octadecylsilyl silica gel (YMC Co., Ltd., Kyoto, Japan; 50 μm), respectively. Spots were detected on TLC (Qingdao Marine Chemical Factory, Qingdao, China) under 254 nm UV light. The NMR spectra were obtained on a Bruker Avance-600 MHz spectrometer (Bruker, Billerica, MA, USA) with tetramethylsilane as an internal standard. HR-ESI-MS spectra were recorded on a Bruker miXis TOF-QII mass spectrometer (Bruker, Billerica, MA, USA). Optical rotations were determined with a Perkin Elmer MPC 500 (Waltham, MA, USA) polarimeter. The UV, IR, and ECD spectra were recorded on a Shimadzu UV-2600 PC spectrometer (Shimadzu, Kyoto, Japan), an IR Affinity-1 spectrometer (Shimadzu, Kyoto, Japan), and a Chirascan circular dichroism spectrometer (Applied Photophysics, Leatherhead Surrey, UK), respectively. The artificial sea salt was a commercial product (Guangzhou Haili Aquarium Technology Company, Guangzhou, China). Cells were disrupted using a high pressure homogenizer NanoGenizer (Genizer LLC, Irvine, CA, USA).

Gas sorption measurements were conducted using a Micrometritics ASAP 2020 system. PXRD was carried out with a Bruker D8-Focus Bragg–Brentano X-ray Powder Diffractometer equipped with a Cu sealed tube (λ=1.54178 Å) at 40 kV and 40 mA. SCXRD was measured on a Bruker Venture CMOS diffractometer equipped with a Cu-K_α sealed-tube X-ray source (λ=1.5406 Å). NMR data were collected on a Mercury 300 spectrometer. Ultraviolet–visible absorption spectra were recorded on a Shimadzu UV-2450 spectrophotometer. ICP-MS data were collected with a Perkin Elmer NexION 300D ICP-MS. TGA was conducted on a TGA-50 (Shimadzu) thermogravimetric analyser. Infrared measurements were performed on a Shimadzu IR Affinity-1 spectrometer. TEM experiments were conducted on a FEI Tecnai G2 F20 ST microscope (America) operated at 200 kV. Field-emission SEM images were collected on the FEI Quanta 600 field-emission SEM (America) at 20 KV.

All solvents used were of reagent grade. MBT and Silver Nitrate(AgNO₃) were used with no further purification. XRD spectrum was recorded for Ag(I)MBT and Au(III)MBT complexes using BRUKER D8 Advance X-ray diffractometer with Cu Kα source (λ = 1.5406 A°). The UV-Vis absorbance spectrum was obtained by measuring the absorbance using Specord/210, analyticjena UV–Vis spectrophotometer. FTIR spectra were recorded using SHIMADZU, IRAffinity1 spectrometer. Solution state ¹HNMR spectra of Ag(I)MBT and Au(III)MBT complexes was recorded by Bruker 500 MHz standard bore (SB) NMR spectrometer equipped with BBO probe head. Electrochemical studies of Ag(I)MBT and Au(III)MBT were carried out employing Zahner Zennium electrochemical workstation.

IR spectra were collected using an IRAffinity-1 spectrometer (Shimadzu company, Kyoto, Japan) for KBr pellets. HRESIMS spectra were determined by a MaXis 4G UHR59 TOFMS spectrometer (Bruker Company, Karlsruhe, Germany). NMR spectra were recorded on a Bruker AVANCE III HD 700 spectrometer with tetramethylsilane (TMS) as the internal standard (Bruker Company, Karlsruhe, Germany). UV spectra were measured using a U-2600 spectrophotometer (Shimadzu Company, Kyoto, Japan).
Semi-preparative HPLC was carried out using the 1260 HPLC system equipped with a G1311C isocratic pump and an Agilent G1315D diode array detector (DAD) (Agilent Company, Santa Clara, CA, USA) using an ODS-A column (10 × 250 mm, 5 μm, YMC company, Kyoto, Japan). Column chromatographs were performed with silica gel (200–300 mesh; Yantai Jiangyou Silica Gel Development company, Yantai, CN), Sephadex LH-20 (GE Healthcare Company, Marlborough, MA, USA), and reversed-phase medium-pressure preparative liquid chromatography (RP-MPLC, Agela Company, Torrance, CA, USA).

The material constituents in their native forms and the developed materials were analyzed by FT-IR spectroscopy using an IRAffinity-1 spectrometer (Shimadzu, Kyoto, Japan). Before the analysis, the samples were homogenously mixed with potassium bromide, compressed into pellets, and then used in that form. The FT-IR spectra were collected in the wavenumber range of 4000–500 cm⁻¹, at a resolution of 4 cm⁻¹, from 100 scan accumulations.