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Smarcb1

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SMARCB1 is a subunit of the SWI/SNF chromatin remodeling complex. It plays a role in the regulation of gene expression and chromatin structure.

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Lab products found in correlation

Smarca4, by Abcam (5 mentions) H3k4me3, by Abcam (3 mentions) Axio imager 2, by Zeiss (2 mentions) Baf155, by Cell Signaling Technology (2 mentions) Baf180, by Fortis Life Sciences (2 mentions) Arid1a, by Abcam (2 mentions) Baf53a, by Abcam (2 mentions) Smarca2, by Cell Signaling Technology (2 mentions) Baf60a, by BD (2 mentions) H3k4me1, by Active Motif (2 mentions) H3k27ac, by Active Motif (2 mentions) Pspc1, by Merck Group (1 mentions) Smarcd1, by Santa Cruz Biotechnology (1 mentions) Flag tag (flag), by Merck Group (1 mentions) β actin, by Abcam (1 mentions)

6 protocols using smarcb1

1

Comprehensive Antibody Validation Protocol

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ARID1A (CST D2A8U, dilution 1:2,000), ARID1B (Abcam ab57461, dilution 1:3,000) FLAG (Sigma‐Aldrich A8592, dilution 1:10,000), Halo (Promega G9211, dilution 1:5,000), PSPC1 (Sigma, SAB4200503, 1: 5,000), SFPQ (Abcam, ab38148, 1:2,000), NONO (Santa Cruz, sc‐166,702 X, 1:8,000), SMARCA4 (ab110641, Abcam, 1: 2,000), SMARCD1 (Santa Cruz, sc‐135,843, 1:1,000), SMARCE1 (Bethyl laboratories, A300‐810A, 1:5,000), SMARCB1 (Abcam, ab58209, 1:1,000), SMARCC1 (CST, 11956S, 1: 2,000), and β‐actin (Abcam ab8224, dilution 1:2,500).
Reddy D., Bhattacharya S., Levy M., Zhang Y., Gogol M., Li H., Florens L, & Workman J.L. (2022). Paraspeckles interact with SWI/SNF subunit ARID1B to regulate transcription and splicing. EMBO Reports, 24(1), e55345.
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2

Immunostaining of SMARCA4 and SMARCB1

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RWPE1;LACZ and RWPE1;SCHLAP1 cells were grown on 0.1% gelatin-coated glass coverslips, fixed in 2% paraformaldehyde, blocked in antibody dilution buffer (goat serum PBS) and immunostained for SMARCA4 (abcam, 1:500 dilution) and SMARCB1 (abcam, 1:400 dilution) overnight at 4 degrees in antibody dilution buffer. Coverslips were washed 3 × 5 minutes in PBS and then stained in secondary antibody (goat anti rabbit, Alexa Fluor 568, 1:500 in antibody dilution buffer) for 45 minutes at RT. Coverslips were again washed 3 × 5 minutes in PBS and mounted with Prolong Gold containing DAPI and imaged on a Zeiss Axio Imager 2.
Raab J.R., Smith K.N., Spear C.C., Manner C.J., Calabrese J.M, & Magnuson T. (2018). SWI/SNF remains localized to chromatin in the presence of SCHLAP1. Nature genetics, 51(1), 26-29.
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3

ChIP-qPCR Assay for Histone Modifications

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ChIP assays were performed according to instructions from Upstate Biotechnology (Lake Placid, NY). For each assay, 50 μg DNA, sheared by a sonication (the DNA fragment size was 200 to 500 bp), was pre-cleared with protein A magnetic beads (Upstate Biotechnology, cat. #16–661) and then 50 μg DNA was precipitated by SMARCB1 (Cambridge, United Kingdom, abcam #12167) and H3K4me3 (active motif #39159). After immunoprecipitation (IP), recovered chromatin fragments were subjected to real-time PCR. IgG control experiments were performed for all ChIPs and incorporated into the IP/Input (1%) by presenting the results as (IP-IgG)/(Input-IgG). ChIP Primers used were as follows: IL6_Promoter-F: TCG TGC ATG ACT TCA GCT TT-3′, IL6_Promoter-R: 5′- TGT GAC GTC CTT TAG CAT GG-3′. Each ChIP PCR was replicated three times and then statistically analyzed.
Choi S.K., Kim M.J, & You J.S. (2020). SMARCB1 Acts as a Quiescent Gatekeeper for Cell Cycle and Immune Response in Human Cells. International Journal of Molecular Sciences, 21(11), 3969.
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4

Chromatin Remodeling Complex Profiling

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SMARCA2 (Cell Signaling 11966) Lot 2; SMARCA4 (Abcam ab110641) - GR150844–42; SMARCB1 (Abcam ab192864) - GR315927–2; BAF53A (Abcam ab3882) - GR95633–1; BAF60A ( BD Biosciences 611728) - 5199785; BAF155 ( Cell Signaling D7F8S) - Lot 2; BAF180 (Bethyl A301–591A) Lot 1; SFPQ ( Genetex GTX114209 ) - 40828; NCL (Bethyl A300–711A) - Lot 1; ARID1A (Abcam ab182560 ) - GR3186289–2; ARID1B (Abcam ab57461) - GR308911–3; ARID2 (ThermoFisher PA5–35857) SF24034426; H3K4me3 (Abcam ab8580); H3K4me1 (Active Motif 39635) 16611004; H3K27ac (Active Motif 39133) 20017049; H3 (epicypher 13–0001) NA gift of Brian Strahl, available from Epicyphyer
Raab J.R., Smith K.N., Spear C.C., Manner C.J., Calabrese J.M, & Magnuson T. (2018). SWI/SNF remains localized to chromatin in the presence of SCHLAP1. Nature genetics, 51(1), 26-29.
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5

Immunostaining of SMARCA4 and SMARCB1

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RWPE1;LACZ and RWPE1;SCHLAP1 cells were grown on 0.1% gelatin-coated glass coverslips, fixed in 2% paraformaldehyde, blocked in antibody dilution buffer (goat serum PBS) and immunostained for SMARCA4 (abcam, 1:500 dilution) and SMARCB1 (abcam, 1:400 dilution) overnight at 4 degrees in antibody dilution buffer. Coverslips were washed 3 × 5 minutes in PBS and then stained in secondary antibody (goat anti rabbit, Alexa Fluor 568, 1:500 in antibody dilution buffer) for 45 minutes at RT. Coverslips were again washed 3 × 5 minutes in PBS and mounted with Prolong Gold containing DAPI and imaged on a Zeiss Axio Imager 2.
Raab J.R., Smith K.N., Spear C.C., Manner C.J., Calabrese J.M, & Magnuson T. (2018). SWI/SNF remains localized to chromatin in the presence of SCHLAP1. Nature genetics, 51(1), 26-29.
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6

Chromatin Remodeling Complex Profiling

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SMARCA2 (Cell Signaling 11966) Lot 2; SMARCA4 (Abcam ab110641) - GR150844–42; SMARCB1 (Abcam ab192864) - GR315927–2; BAF53A (Abcam ab3882) - GR95633–1; BAF60A ( BD Biosciences 611728) - 5199785; BAF155 ( Cell Signaling D7F8S) - Lot 2; BAF180 (Bethyl A301–591A) Lot 1; SFPQ ( Genetex GTX114209 ) - 40828; NCL (Bethyl A300–711A) - Lot 1; ARID1A (Abcam ab182560 ) - GR3186289–2; ARID1B (Abcam ab57461) - GR308911–3; ARID2 (ThermoFisher PA5–35857) SF24034426; H3K4me3 (Abcam ab8580); H3K4me1 (Active Motif 39635) 16611004; H3K27ac (Active Motif 39133) 20017049; H3 (epicypher 13–0001) NA gift of Brian Strahl, available from Epicyphyer
Raab J.R., Smith K.N., Spear C.C., Manner C.J., Calabrese J.M, & Magnuson T. (2018). SWI/SNF remains localized to chromatin in the presence of SCHLAP1. Nature genetics, 51(1), 26-29.
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