Log In Sign up
The largest database of trusted experimental protocols

Accuri

Manufactured by BD
Visit Supplier
Sourced in United States, Italy, Germany, United Kingdom

The BD Accuri is a compact flow cytometer designed for research and clinical applications. It provides automated sample acquisition, analysis, and data management capabilities. The instrument utilizes laser-based detection technology to measure and analyze various parameters of individual cells or particles within a sample.

Automatically generated - may contain errors

Lab products found in correlation

Accuri c6, by BD (13 mentions) Facscalibur, by BD (7 mentions) Facsaria, by BD (5 mentions) Lsrfortessa, by BD (5 mentions) Sytox green, by Thermo Fisher Scientific (4 mentions) C6 flow cytometer, by BD (4 mentions) Propidium iodide, by Merck Group (4 mentions) Facscanto, by BD (4 mentions) Fcap software v3.0, by BD (4 mentions) 7 aad, by Thermo Fisher Scientific (3 mentions) Fitc annexin 5 apoptosis detection kit, by BD (3 mentions) Accuri c6 plus software, by BD (3 mentions) Bovine serum albumin (bsa), by Merck Group (3 mentions) Cflow sampler software, by BD (3 mentions) Lsrfortessa x 20, by BD (3 mentions) Aldefluor assay, by STEMCELL (3 mentions) Penicillin, by Lonza (3 mentions) Streptomycin, by Lonza (3 mentions) H2dcfda, by Thermo Fisher Scientific (3 mentions) Fc block, by BD (3 mentions)

Close spelling variants of this product

BD Accuri C6 Plus (765 citations) BD Accuri C6 instrument (36 citations) BD Accuri C5 flow cytometer (22 citations) BD Accuri CFlow software (16 citations) BD Accuri 6 plus (8 citations) Accuri 6 cytometer (8 citations) BD Accuri C6 Analysis (7 citations) BD Accuri™ C6 Plus Cell Analyzer (7 citations) Accuri C6 Personal Flow Cytometer (4 citations) BD Accuri 6C Plus (4 citations)

258 protocols using accuri

1

Transfection, Cell Cycle, and Apoptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
BCs were seeded in 12 well-plate at a density of 5×10 4 , 0.5 µg GFP-LC3 (addgene) or pmCherry-1 CHOP promoter (-649/+136) (addgene #36035) plasmids transfected by using Fugene HD reagent. Following 48 h transfection, 24 h drug (2 µM) applied to the culture medium. The uorescence of green uorescence and mCherry were detected by the BD Accuri cell ow cytometer via FL-1 and FL-3 channels, respectively. 1x10 4 cells/samples were analyzed for each condition.
2.6. Cell cycle analysis 5 × 10 4 cells/well were seeded in 12-well plates and treated with 0-2.5 µM atiprimod for 24 h. Following trypsinization, the cells were xed with 70% and incubated at -20°C. Discarding ethanol by centrifugation, cells were stained by PI/RNase staining buffer for 30 min and 1 × 10 4 cells were analyzed by ow cytometer (BD Accuri, Becton Dickinson USA) via using C6 plus software. 2.7. Apoptotic death determination by Annexin V/ PI staining 1x10 5 cells/Petri MDA-MB-231 and MDA-MB-468 cells were seeded into 60 mm Petri dishes and treated with a selected dose of atiprimod for 24 h. Prior to drug exposure, cells were trypsinized, pellets were applied with FITC Annexin V Apoptosis Detection Kit and ow cytometric analysis was performed with cells were analyzed by ow cytometer (BD Accuri, Becton Dickinson USA) via using C6 plus software.
Çoker-Gürkan A., Can E., Sahin S., Yerlikaya P.O., & Arısan E.D. (2021). Atiprimod Triggered Apoptotic Cell Death via Acting on PERK/eIF2a/ATF4/CHOP and STAT3/NF-KB axis in MDA-MB-231 and MDA-MB-468 Breast Cancer Cells.
+ Open protocol
+ Expand
2

Transfection, Cell Cycle, and Apoptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
BCs were seeded in 12 well-plate at a density of 5×10 4 , 0.5 µg GFP-LC3 (addgene) or pmCherry-1 CHOP promoter (-649/+136) (addgene #36035) plasmids transfected by using Fugene HD reagent. Following 48 h transfection, 24 h drug (2 µM) applied to the culture medium. The uorescence of green uorescence and mCherry were detected by the BD Accuri cell ow cytometer via FL-1 and FL-3 channels, respectively. 1x10 4 cells/samples were analyzed for each condition.
2.6. Cell cycle analysis 5 × 10 4 cells/well were seeded in 12-well plates and treated with 0-2.5 µM atiprimod for 24 h. Following trypsinization, the cells were xed with 70% and incubated at -20°C. Discarding ethanol by centrifugation, cells were stained by PI/RNase staining buffer for 30 min and 1 × 10 4 cells were analyzed by ow cytometer (BD Accuri, Becton Dickinson USA) via using C6 plus software. 2.7. Apoptotic death determination by Annexin V/ PI staining 1x10 5 cells/Petri MDA-MB-231 and MDA-MB-468 cells were seeded into 60 mm Petri dishes and treated with a selected dose of atiprimod for 24 h. Prior to drug exposure, cells were trypsinized, pellets were applied with FITC Annexin V Apoptosis Detection Kit and ow cytometric analysis was performed with cells were analyzed by ow cytometer (BD Accuri, Becton Dickinson USA) via using C6 plus software.
Coker-Gurkan A., Can E., Sahin S., Obakan-Yerlikaya P, & Arisan E.D. (2021). Atiprimod triggered apoptotic cell death via acting on PERK/eIF2α/ATF4/CHOP and STAT3/NF-ΚB axis in MDA-MB-231 and MDA-MB-468 breast cancer cells. Molecular biology reports, 48(6).
+ Open protocol
+ Expand
3

Sourdough Fermentation Kinetics

Check if the same lab product or an alternative is used in the 5 most similar protocols
Competition between GFP-labelled and unlabeled strains and single strain fermentations kinetics were performed in a sourdough synthetic medium (SSM) that was adapted from [64] to better mimic the average composition of sourdoughs. The SSM contained, per liter: wheat peptone, 24 g; MgSO 4 .7H 2 0, 0. (Accuri, BD Biosciences). For competition experiments, 5.10 5 cells/mL of each strain were then inoculated in 15 mL SSM medium. For single strain analysis of fermentation kinetics, 10 6 cells/mL of pre-culture were inoculated in 15 mL SSM media.
Fermentations were carried out at 24 °C with constant magnetic stirring (300 rpm) in 20 mL glass tubes closed with a filter tip to allow release of CO 2 . Fermentations were monitored during 24 h for CO 2 release, by measuring weight loss every 40 min using an automated robotic system [65] .
At the end of fermentation, cultures were centrifuged and pellets were resuspended in PBS for flow cytometry analysis (C6 cytometer, Accuri, BD Biosciences). Population size and cell viability were determined as described in [66] (link). Relative fitness of tetraploid vs diploid strains was estimated based on the proportion of GFP-labelled vs unlabelled strains in mixed cultures. GFP fluorescence (excitation 488 nm, emission 530 nm) was collected in the FL1 channel.
Bigey F., Segond D., Friedrich A., Guézenec S., Bourgais A., Huyghe L., Agier N., Nidelet T., & Sicard D. (2020). Evidence for two main domestication trajectories inSaccharomyces cerevisiaelinked to distinct bread-making processes.
+ Open protocol
+ Expand
4

Cellular Characterization and Calcium Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
For CSC analysis, cells were collected after 7 days of BCF or Sham treatment, washed with PBS, and incubated with CD44-APC and ESA-PE for 20 min followed by examining marker positive population (CD44high,ESAhigh) using BD Accuri. For calcium assay, Fluo-4 dye was added to the media of cells that were previously exposed to Sham or BCF for 2 h and 30 mins. After Fluo-4 addition, cells were exposed to Sham or BCF 30 mins. Cells were kept at room temperature for 30 min, washed twice with HBSS, scraped off from dish, dissociated by gentle pipetting, and ran on BD Accuri.
Sharma S., Wu S.Y., Jimenez H., Xing F., Zhu D., Liu Y., Wu K., Tyagi A., Zhao D., Lo H.W., Metheny-Barlow L., Sun P., Bourland J.D., Chan M.D., Thomas A., Barbault A., D'Agostino R.B., Whitlow C.T., Kirchner V., Blackman C., Pasche B, & Watabe K. (2019). Ca2+ and CACNA1H mediate targeted suppression of breast cancer brain metastasis by AM RF EMF. EBioMedicine, 44, 194-208.
+ Open protocol
+ Expand
5

Annexin V and Cell Cycle Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
For annexin V assays, 500,000 cells plated at 1 M ml−1 and treated with drug for indicated timepoints and doses were harvested on ice and washed twice in 2.5% FBS/PBS. Of 7-AAD, 2.5 μl and 2.5 μl of FITC-annexin V (640922, BioLegend) were added. Cells were incubated for 15 min at room temperature, then immediately measured on a BD Accuri. Gates were drawn based on single-stain and no-stain controls. For cell cycle and TUNEL analysis, cells plated at 1 M ml−1 were treated with drug for indicated timepoints and doses and pulsed with 10 μM ethynyl-EdU (C10424, Thermo) for 2 h before harvesting on ice. One million cells were counted and washed in 2.5% FBS/PBS. Cells were resuspended at 10 M ml−1 and fixed in 4% paraformaldehyde, washed and permeabilized in 0.5% Triton X-100/PBS. Fixed and permeabilized cells were washed and labelled with BrdUTP using terminal deoxynucleotidyl transferase (556405, BD) for 60 min at 37 °C, rinsed and then labelled with AlexaFluor 647-azide (C10424, Thermo) for 30 min at room temperature in the dark. After washes, the sample was incubated with 2 μl 7-AAD and 5 μl RNAseA for 30 min at room temperature in the dark, washed and measured on a BD Accuri. Gates were drawn based on single-stain and no-stain controls and kept constant across conditions.
van Mierlo C.P., Darby N.J, & Creighton T.E. (1992). The partially folded conformation of the Cys-30 Cys-51 intermediate in the disulfide folding pathway of bovine pancreatic trypsin inhibitor.. Proceedings of the National Academy of Sciences of the United States of America, 89(15), 6775-6779.
+ Open protocol
+ Expand
6

Foxp3 Expression Analysis in A549 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
A549 cells were seeded at 3.5×105 per well in 6-well plates, incubated overnight at 37°C and transfected with siRNA-Foxp3 as described above. A549 cells were collected and counted, and 1×106 cells were suspended in PBS (100 µl total volume). The cells were fixed with 4% paraformaldehyde at 4°C for 1 h, and then treated with 0.1% saponin (Sigma-Aldrich; Merck KGaA) and phycoerythrin (PE) anti-mouse Foxp3 monoclonal antibody (cat. no. M300F8-09A; 5 µl/test; Tianjin Sungene Biotech, Co., Ltd., Tianjin, China) at 4°C for 1 h. The cells were washed twice with PBS and resuspended in 2% paraformaldehyde (Sigma-Aldrich; Merck KGaA). A total of 105 events were assessed using BD Accuri (BD Biosciences, Franklin Lakes, NJ, USA), and the data were analyzed using the BD Accuri C6 Software version 6.0.
Li Y., Yang W., Wu B., Liu Y., Li D., Guo Y., Fu H, & Li Y. (2017). KDM3A promotes inhibitory cytokines secretion by participating in TLR4 regulation of Foxp3 transcription in lung adenocarcinoma cells. Oncology Letters, 13(5), 3529-3537.
+ Open protocol
+ Expand
7

Gene Reporter Assay in mESCs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
mESCs with a Tol2 transposon-integrated reporter construct containing 2x RAR binding site, minimal Hsp68 promoter, and GFP were generated previously [5 (link)]. CAGGS-dCas9 was added to these cells using the Tol2 transposon system as described above. sgRNAs were added to the cells when noted using the Tol2 transposon system. Primer sequences used to clone these sgRNAs are listed in S1 Table. Flow cytometry was performed using BD Accuri and data was analyzed on BD Accuri software.
Barkal A.A., Srinivasan S., Hashimoto T., Gifford D.K, & Sherwood R.I. (2016). Cas9 Functionally Opens Chromatin. PLoS ONE, 11(3), e0152683.
+ Open protocol
+ Expand
8

Quantifying ACKR2 Ligand Binding Affinities

Check if the same lab product or an alternative is used in the 5 most similar protocols
To determine CCL2 binding levels for ACKR2-WT and ACKR2-V41A, cells were incubated with conjugated CCL2-AF647 (ALMAC, Cat# CAF-02-D-2) at 37 °C for 45 minutes at different concentrations ranging between 100 to 750 nM. After two washes receptor expression was analyzed on a BD Accuri. To determine the levels of ACKR2-WT and ACKR2-V41A receptor expression, cells were stained with anti-CD16/32 (Fc block) on ice for 20 minutes. After two washes, cells were stained for 30 minutes at 37 °C with anti-human ACKR2 polyclonal IgG antibodies, washed twice, and then stained for 30 minutes at 4 °C with anti-human IgG conjugated with PE. In the CCL2 competitive binding assay levels for ACKR2-WT and ACKR2-V41A, cells were incubated with unconjugated CCL2 (PEPROTECH, Cat #300-04100UG) at 37 °C for 45 minutes. After two washes and an Fc block as described above, cells were stained for 30 minutes at 37 °C with anti-human ACKR2 polyclonal IgG antibodies, washed twice, and then stained for 30 minutes at 4 °C with anti-human IgG conjugated with PE. After two washes, levels of binding of the ACKR2 antibody was analyzed in BD Accuri.
CCL4 (PEPROTECH, Cat #300-.9) competition binding assay was performed following the procedures above. Conjugated CCL19-AF647 (ALMAC, Cat #CAF-06-A-01) binding analyses were completed using the same methods used for CCL2 binding assay.
Murcia J.D., Weinert A., Freitas C.M., Arens D.K., Ferrel M.N., Grose J.H., Ridge P.G., Wilson E., Kauwe J.S, & Weber K.S. (2020). Atypical chemokine receptor ACKR2-V41A has decreased CCL2 binding, scavenging, and activation, supporting sustained inflammation and increased Alzheimer’s disease risk. Scientific Reports, 10, 8019.
+ Open protocol
+ Expand
9

Yeast Display Binding Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Binding of yeast-displayed hVISTA (Phe33-Ala194, Uniprot #Q9H7M9) to soluble SG7, BMS767, and VSTB112 antibodies (Supplementary Fig. S5A, S5B) was measured. Titrations of antibody were incubated with yeast in PBS/BSA (pH 6.0 or pH 7.4) at 4 °C for 12 h to allow interactions to reach equilibrium. Binding signal was measured on a BD Accuri and analyzed on FlowJo and GraphPad Prism as before. PBS/BSA at pH 6.0 was prepared by titrating in hydrochloric acid to standard PBS + 1 mg/mL BSA. hVISTA and mVISTA mutants were displayed on yeast for single clone analysis (Fig. 3c,e). Mutants based on wild-type hVISTA ECD (Phe33-Ala194, Uniprot #Q9H7M9) or wild-type mVISTA ECD (Phe33-Ala191, Uniprot # Q9D659) were induced for surface expression on yeast as described previously. Yeast-displayed clones were incubated with a concentration of each antibody at its estimated Kd value (SG7: 300 pM; BMS: 3 nM; or VSTB: 3 nM) in PBS/BSA at pH 7.4 (SG7, VSTB112) or pH 6.0 (BMS767) at 4 °C for 12 h to allow interactions to reach equilibrium. Binding signal was measured on a BD Accuri and analyzed on FlowJo and GraphPad Prism as before.
Mehta N., Maddineni S., Kelly R.L., Lee R.B., Hunter S.A., Silberstein J.L., Parra Sperberg R.A., Miller C.L., Rabe A., Labanieh L, & Cochran J.R. (2020). An engineered antibody binds a distinct epitope and is a potent inhibitor of murine and human VISTA. Scientific Reports, 10, 15171.
+ Open protocol
+ Expand
10

Flow Cytometric Analysis of Podoplanin and S100A13

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells suspended in PBS (5 × 106/mL, 100 μL) were incubated with the primary antibodies control IgG or anti-podoplanin Ab (NZ-1) at 5–10 μg/mL or the recombinant proteins (hFc2 or hCLEC-2-hFc2) at 100 ∞g/mL. After washing with PBS, these cells were resuspended with 100 ∞L of PBS and stained with 3 ∞L of secondary antibodies (anti-hamster IgG or anti-human IgG) conjugated with fluorescein isothiocyanate (FITC) for 15 min.
Where indicated, cells suspended in modified Tyrode’s buffer supplemented with vehicle, 1 mM CaCl2, or 1 mM EDTA were incubated with or without GST-tagged S100A13 recombinant protein at 20 ∞g/mL. Then the unbound recombinant proteins were washed with PBS and the binding of S100A13 protein to the cells was detected by 10 ∞g/mL anti-S100A13 antibody (63Y, Santa Cruz Biotechnology, Inc.) and anti-mouse IgG conjugated with Alexa Fluor 488.
Surface expression of endogenous S100A13 was also analyzed by flow cytometry. CASMCs pretreated with or without H2O2 as described above were incubated with 10 ∞g/mL control mouse IgG or anti-S100A13 antibody (63Y), followed by Alexa Fluor 488-conjugated anti-mouse IgG.
The stained cells were immediately analyzed using a FACScan or BD Accuri (BD Biosciences). The data were recorded and analyzed using CellQuest software or BD Accuri CFlow software.
Inoue O., Hokamura K., Shirai T., Osada M., Tsukiji N., Hatakeyama K., Umemura K., Asada Y., Suzuki-Inoue K, & Ozaki Y. (2015). Vascular Smooth Muscle Cells Stimulate Platelets and Facilitate Thrombus Formation through Platelet CLEC-2: Implications in Atherothrombosis. PLoS ONE, 10(9), e0139357.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!